Actin distribution in mitotic apparatus of somatic embryo cells of Norway spruce (Picea abies (L.) Karst.)

Cenklová, Věra; Binarová, Pavla; Havel, L.
BIOLOGIA PLANTARUM 46 [2]: 167-174, 2003

Klíčová slova: cytoskeleton; embryogenesis of spruce; mitosis
Abstrakt: F-actin distribution in cells of embryogenic suspension culture of Norway spruce (Picea abies (L.) Karst.) was studied with specific focus to mitosis. Actin was present in dividing cells of embryo head during whole mitosis. Transient localization of actin microfilaments with preprophase band of microtubules was observed. Weak actin staining occurred with non kinetochor microtubular fibers in metaphase spindle, while actin was absent in the area of shortening microtubular kinetochore fibers in early anaphase. Abundant actin microfilaments formed anaphase spindle in equatorial level between separating sister chromatids later in anaphase and were present in phragmoplast area in telophase. The possible role of actin in cell division and cytokinesis of higher plants is discussed.
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