Chromosome sorting in tetraploid wheat and its potential for genome analysis

Kubaláková, Marie; Kovářová, Pavlína; Suchánková, Pavla; Číhalíková, Jarmila; Bartoš, Jan; Lucretti, S.; Watanabe, N.; Kianian, S. F.; Doležel, Jaroslav
GENETICA 170: 823-829, 2005

Klíčová slova: Flow cytometry; chromosome sorting; wheat
Abstrakt: This study evaluates the potential of flow cytometry for chromosome sorting in durum wheat (Triticum turgidum Desf. var. durum,). Histograms of fluorescence intensity obtained after the analysis of DAPI-stained chromosomes consisted of three peaks. Of these, one represented chromosome 3B, a small peak corresponded to chromosomes 1A and 6A, and a large peak represented the remaining eleven chromosomes. Flow karyotyping in double ditelosomic lines of durum wheat revealed that the lines facilitated sorting of any arm of the wheat A- and B-genome chromosomes. We have demonstrated that large insert libraries can be created from DNA purified using flow cytometry. This study considerably expands the potential of flow cytogenetics for use in wheat genomics and opens the possibility of sequencing the genome of this important crop one chromosome arm at a time.
Autoři z ÚEB: Jan Bartoš, Jaroslav Doležel, Pavla Suchánková