Nowicka A., Ferkova L., Said M., Kovacik M., Pecinka A.
Book of Abstracts - Cytogenetics Meeting 2021- Chromosome Biology in context of Evolution and Plant Breeding - September 27-28 - Görlitz - Germany : , 2021

Klíčová slova:
Abstrakt: The endosperm is a triploid seed tissue unique to flowering plants. In cereals, endosperm expands and forms the largest nutrient and energy-rich parts of the seed. The role of higher-order chromatin organization in regulating the endosperm genome was long ignored due to technical hindrance. Using flow-sorted nuclei, we analyze the nuclear structure and chromatin organization in barley endosperm from fertilization until the seed ripening. We found that developing and differentiating barley endosperm tissues undergo a phase of active nuclei division followed by two rounds of endoreduplication. The endoreduplicated endosperm nuclei had changed morphology, decondensed centromeric and telomeric chromatin, and reduced sister chromatids alignment in age-dependent manner. This coincided with a progressive depletion of centromere-specific histone H3 (CENH3) and reduced genome stability. In summary, our data open a new route towards understanding chromatin changes accompanying endoreduplication and endosperm development. Funding: This work was supported by the Czech Science Foundation grants 18-112197S and 21-02929S.
DOI:
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Autoři z ÚEB: Martin Kovačik, Anna Nowicka, Aleš Pečinka, Mahmoud Said