Localization of low-copy DNA sequences on mitotic chromosomes by FISH

Karafiátová, M., Bartoš, J., Doležel, J.
Plant Cytogenetics: Methods and Protocols : 49-64, 2016

Klíčová slova: Cell cycle synchronization , Cytogenetic mapping , Fluorochrome , Low-copy probe , Mitotic metaphase chromosomes , Probe purifi cation , Post-fi xation , Quantum yield
Abstrakt: Fluorescence in situ hybridization (FISH) is a widely used method to localize DNA sequences on mitotic and meiotic chromosomes and interphase nuclei. It was developed in early 1980s and since then it has contributed to numerous studies and important discoveries. Over the decades, the protocol was modifi ed for ease of use, allowing for localizing multiple probes simultaneously and increasing its sensitivity and specifi city. Despite the continuous improvements, the ability to detect short single-copy sequences of only a few kilobases or less, such as genes, remains limited. Here, we provide a detailed protocol for detection of short, single- or low-copy sequences on plant mitotic metaphase chromosomes.
DOI: 10.1007/978-1-4939-3622-9_5
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