O-Glucosylation of cis-Zeatin in Maize. Characterization of Genes, Enzymes, and Endogenous Cytokinins

Veach, Y. K.; Martin, R. C.; Mok, D. W. S.; Malbeck, Jiří; Vaňková, Radomíra; Mok, M. C.
PLANT PHYSIOLOGY 131 [3]: 1374-1380, 2003

Klíčová slova: Cis-cytokinin; O-glucosylation; Zea Mays
Abstrakt: Trans-Zeatin is a major and ubiquitous cytokinin in higher plants. cis-Zeatin has traditionally been viewed as an adjunct with low activity and rare occurrence. Recent reports of cis-zeatin and its derivatives as the predominant cytokinin components in some plant tissues may call for a different perspective on cis-isomers. The existence of a maize (Zea mays) gene (cisZOG1) encoding an O-glucosyltransferase specific to cis-zeatin (R.C. Martin, M.C. Mok, J.E. Habben, D.W.S. Mok [2001] Proc Natl Acad Sci USA 98: 5922-5926) lends further support to this view. Results described here include the isolation of a second maize cisZOG gene, differential expression of cisZOG1 and cisZOG2, and identification of substantial amounts of cis-isomers in maize tissues. The open reading frame of cisZOG2 has 98.3
Autoři z ÚEB: Jiří Malbeck, Radomíra Vanková