Production of microgram amounts of DNA from flow-sorted plant chromosomes

Suchánková, Pavla; Šimková, Hana; Svensson, J.T.; Hřibová, Eva; Condamine, P.; Bhat, P.R.; Bartoš, Jan; Šafář, Jan; Close, T.J.; Doležel, Jaroslav
CHROMOSOME RESEARCH 15 [2]: 4-5, 2007

Klíčová slova: flow-sorted chromosomes; subgenomic DNA libraries
Abstrakt: Chromosome sorting using flow cytometry simplifies the analysis of complex genomes by dissecting them to small parts. As DNA of flowYsorted chromosomes is intact, they are suitable for a range of applications including the construction of largeYinsert subgenomic DNA libraries, physical mapping using PCR, targeted isolation of molecular markers and molecular cytogenetic mapping.
Autoři z ÚEB: Jan Bartoš, Jaroslav Doležel, Jan Šafář, Hana Šimková, Pavla Suchánková