Čeština
English24-Epibrassinolide at Subnanomolar Concentrations Modulates Growth of Mouse Hybridoma Cells
Franěk, František; Kohout, Ladislav; Eckschlager, T.
Conference of Isoprenoids 20. Liberec : , 2003
Keywords: Brassinolides; cell division
Abstract: Brassinolides are known to stimulate plant growth and to posses antistresiTactivities in plants. This work was aimed at exploring possible beneficial effects of 24-epibrassinolide on cultured mammalian cells. A mouse hybridoma, i.e. a hybrid ymphocyte cell line, was cultured either in standard serum-free medium, or in diluted medium in which the cell grew inder nutritional stress. Steady-state parameters of semicon-finuous cultures conducted at 24-epibrassinolide concentra-ions from 10~16 M to 1CT9 M were evaluated. Typical effects of he agent that were observed both in standard and in diluted i [media were suppression of intracellular antibody level, increa- / ,ed value of mitochondrial membrane potential, higher frac- / ion of cells in the G(/Gl phase and lower fraction of cells in / he S phase. Alleviation of nutritional stress was observed in j (cultures conducted in diluted media. Viable cell density was j ignificantly higher at 24-epibrassinolide concentrations 10~13[ knd 10~12 M. Results of this exploratory study show that thej plant hormone 24-epibrassinolide may induce alterations of he cell division mechanism of the energetic metabolism, and of secreted protein synthesis in a mammalian cell line.
DOI:
IEB authors: bývalý zaměstnanec
Conference of Isoprenoids 20. Liberec : , 2003
Keywords: Brassinolides; cell division
Abstract: Brassinolides are known to stimulate plant growth and to posses antistresiTactivities in plants. This work was aimed at exploring possible beneficial effects of 24-epibrassinolide on cultured mammalian cells. A mouse hybridoma, i.e. a hybrid ymphocyte cell line, was cultured either in standard serum-free medium, or in diluted medium in which the cell grew inder nutritional stress. Steady-state parameters of semicon-finuous cultures conducted at 24-epibrassinolide concentra-ions from 10~16 M to 1CT9 M were evaluated. Typical effects of he agent that were observed both in standard and in diluted i [media were suppression of intracellular antibody level, increa- / ,ed value of mitochondrial membrane potential, higher frac- / ion of cells in the G(/Gl phase and lower fraction of cells in / he S phase. Alleviation of nutritional stress was observed in j (cultures conducted in diluted media. Viable cell density was j ignificantly higher at 24-epibrassinolide concentrations 10~13[ knd 10~12 M. Results of this exploratory study show that thej plant hormone 24-epibrassinolide may induce alterations of he cell division mechanism of the energetic metabolism, and of secreted protein synthesis in a mammalian cell line.
DOI: