Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes.

Potocký M., Bezvoda R., Pejchar P.
In: Plant Cell Morphogenesis eds. Cvrčková F. Žárský V. (Humana New York) 1992: 359-365, 2019

Keywords: Antisense oligodeoxynucleotide, Pollen tub,e Gene knockdown, Tip growth
Abstract: Specific gene knockdown mediated by the antisense oligodeoxynucleotides (AODNs) strategy emerged as a rapid and effective tool for probing gene role in plant cells, particularly tip-growing pollen tubes. Here, we describe the protocol for the successful employment of AODN technique in growing tobacco pollen tubes, covering AODN design, application, and analysis of the results. We also discuss the advantages and drawbacks of this method.
DOI: 10.1007/978-1-4939-9469-4_24 IEB authors: Přemysl Pejchar, Martin Potocký