Čeština
EnglishAssessment of ploidy stability of the somatic embryogenesis process in Quercus suber L. using flow cytometry
Loureiro, J.; Pinto, G.; Lopes, T.; Doležel, Jaroslav; Santos, C.
PLANTA 221: 815-822, 2005
Keywords: Flow cytometry; ploidy stability; nuclear DNA content
Abstract: Flow cytometry analyses were used to verify the ploidy stability of Quercus suber L. somatic embryogenesis process. Leaf explants of cork oak trees were inoculated on MS medium with 2,4-D and zeatin. Calluses with embryogenic structures were transferred to fresh MS medium without growth regulators and somatic embryo evolution was followed. Flow cytometry was employed to estimate DNA ploidy levels and nuclear DNA content of somatic embryos and leaves from mother plants. No significant differences (P 0.05) were detected among embry-os, and between the embryos and the mother plants. Also, after conversion of these embryos, no significant morphological differences were observed among the somatic embryoderived plants. These results indicate that embryo cultures and converted plantlets were stable with regard to ploidy level.
DOI:
IEB authors: Jaroslav Doležel
PLANTA 221: 815-822, 2005
Keywords: Flow cytometry; ploidy stability; nuclear DNA content
Abstract: Flow cytometry analyses were used to verify the ploidy stability of Quercus suber L. somatic embryogenesis process. Leaf explants of cork oak trees were inoculated on MS medium with 2,4-D and zeatin. Calluses with embryogenic structures were transferred to fresh MS medium without growth regulators and somatic embryo evolution was followed. Flow cytometry was employed to estimate DNA ploidy levels and nuclear DNA content of somatic embryos and leaves from mother plants. No significant differences (P 0.05) were detected among embry-os, and between the embryos and the mother plants. Also, after conversion of these embryos, no significant morphological differences were observed among the somatic embryoderived plants. These results indicate that embryo cultures and converted plantlets were stable with regard to ploidy level.
DOI: