Influence of Antiviral Factor on Tobacco Mosaic Virus RNA Biosynthesis in Tobacco

Šindelářová, Milada; Šindelář, Luděk
BIOLOGIA PLANTARUM 46 [1]: 95-100, 2003

Keywords: Nicotiana tabacum; antiviral factor; TMV
Abstract: An antiviral factor (AVF) was separated by removing virus particles from extracts of tobacco mosaic virus (TMV) infected leaves using calcium phosphate gel and by column chromatography on DEAE cellulose. AVF was not found in the extracts from healthy plants. The AVF restricted the virus infectivity "in vivo" and significantly decreased the activity of key enzymes of metabolic pathways tending to the purine and pyrimidine nucleotides biosynthesis of viral-RNA (glucose-6-phosphate dehydrogenase, ribonucleases, phosphomonoesterase and phosphodiesterase). No inhibition of these enzymes was observed "in vitro" when the effect of different concentrations of AVF (0.25 - 250 mug cm(-3)) was examined.
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