Laboratory of Mass Spectrometry
Laboratory of Mass Spectrometry – service department
The laboratory was established in 2007 by separation from the larger research division, in which the laboratory team worked as an analytical unit. From the beginning the laboratory was defined as a service centre performing special instrumental analyses and development of new analytical methods. The main task of the laboratory was to provide these services both to other research groups at the home institution and collaborate with other research institutes and universities as well. Currently, we provide analytical services to other entities in the Czech Republic and abroad.
The main part of the laboratory equipment consists of two LC-MS systems, one GC-MS system and one HPLC instrument with fraction collector device.
LC-MS Ion-Trap system
It is an assembly of an ion-trap (IT) tandem mass spectrometer with a high-performance liquid chromatograph, an auto sampler with a cooled sample tray and alternative optical diode array detector. The optical detector is compatible with other LC-MS system. Mass spectrometer operates with unit resolution and allows for repeated subsequent fragmentation (MRM)
LC-MS Triple-Quad system
It is an assembly of a triple-quad (QQQ) tandem mass spectrometer with two high-performance liquid chromatographs and auto sampler with a cooled sample tray. It can be connected with an optical detector of the previous system.
Triple quadrupole provides MS / MS spectra and depending on its setting can monitor precursors of the selected fragment, neutral loss etc. Due hyperbolic cross of quadrupole rods the mass spectrometer enables to carry out the analysis using a high resolution mode (H-SRM) and accurate mass measurements with an accuracy of 2 ppm. This accuracy helps determine the molecular formula of unknown substances.
GC-MS Ion-Trap system
It is an assembly of an ion-trap (IT) tandem mass spectrometer with two-channel gas chromatograph with digital control flow of the carrier gas and two injector ports (SSL and PTV). The mass spectrometer is equipped with an electron (EI) and chemical (CI +, Cl) ionization and allows up to five times repeated fragmentation. Due the variable programmable auto sampler allows analysing both liquid and gaseous samples.
HPLC Agilent 1200 with fraction collector
This assembly of a high performance liquid chromatograph allows both the classical HPLC analysis (with UV and/or fluorescent detection) and collect chromatographic fractions. The fractions can be used for further analysis (e.g. mass spectrometry).
Other laboratory equipment for sample preparation
Microbalance Mettler Toledo XP26
Analytical balance Sartorius H110
Rotating vacuum concentrator Christ RVC
Rotating evaporator Büchi
Vacuum SPE manifold
Ultrapure water purification system Millipore RiOs 5 / Milli-Q Synthesis A10
Quantitative determination of specific organic substances in plant and/or other matrix by methods developed on our own.
Examples of analyzes of plant and other matrix:
- Cytokinins including zeatin, isopentenyladenine, benzylaminopurine and their derivatives
- Abscisic acid and indolacetic acid
- Polyamines (diaminopropane, putrescine, cadaverine, spermidine, norspermidine, spermine, thermospermine, norspermine)
- Phenolic acids (gallic, para-hydroxybenzoic, meta-hydroxybenzoic, salicylic, pyrocatechuic, protocatechuic, gentisic, ortho-coumaric, para-coumaric, vanillic, syringic, sinapic, cinnamic, caffeic, ferulic, anisic, chlorogenic)
Development of a method according to the customer requirements and subsequent analysis of supplied samples
Determination of unknown substances
Based on the determination of the accurate mass of ions, isotopic profile, fragmentation spectra and other data, it is possible to determine unknown substances with a certain probability.
- MOLECULAR PLANT-MICROBE INTERACTIONS 25 1073-1083 2012 Fulltext: PDF link on intranet only
Cytokinin profiles of AtCKX2-overexpressing potato plants and the impact of altered cytokinin homeostasis on tuberization in vitroJOURNAL OF PLANT GROWTH REGULATION 31 460-470 2012 Fulltext: PDF link on intranet only
Distribution, biological activities, metabolism, and the conceivable function of cis-zeatin-type cytokinins in plantsJOURNAL OF EXPERIMENTAL BOTANY 62 2827-2840 2011 (External fulltext)
Changes in cytokinin levels and metabolism in tobacco (Nicotiana tabacum L.) explants during in vitro shoot organogenesis induced by trans-zeatin and dihydrozeatinPLANT GROWTH REGULATION 65 427-437 2011 Fulltext: PDF link on intranet only
Local induction of senescence by darkness in Cucurbita pepo (zucchini) cotyledons or the primary leaf induces opposite effects in the adjacent illuminated organPLANT GROWTH REGULATION 65 459-471 2011 Fulltext: PDF link on intranet only
Perioxidases play important roles in abscisic acid (ABA)-simulating photosystem II (PSII) thermostabilty of apple tree rootstock leavesAFRICAN JOURNAL OF BIOTECHNOLOGY 10 15891-15900 2011 Fulltext: PDF link on intranet only
- PLANT GROWTH REGULATION 65 23-34 2011
The auxin influx carriers AUX1 and LAX3 are involved in auxin-ethylene interactions during apical hook development in Arabidopsis thaliana seedlings.DEVELOPMENT 137 597–606 2010 Fulltext: PDF link on intranet only
- JOURNAL OF EXPERIMENTAL BOTANY 63 (13) 3589–3598 2010 Fulltext: public PDF link
Comparison of hormonal responses to heat, drought and combined stress in tobacco plants with elevated proline contentJOURNAL OF PLANT PHYSIOLOGY 167 1360-1370 2010