Laboratory of Mass Spectrometry
Laboratory of Mass Spectrometry – service department
The laboratory was established in 2007 by separation from the larger research division, in which the laboratory team worked as an analytical unit. From the beginning the laboratory was defined as a service centre performing special instrumental analyses and development of new analytical methods. The main task of the laboratory was to provide these services both to other research groups at the home institution and collaborate with other research institutes and universities as well. Currently, we provide analytical services to other entities in the Czech Republic and abroad.
The main part of the laboratory equipment consists of two LC-MS systems, one GC-MS system and one HPLC instrument with fraction collector device.
LC-MS Ion-Trap system
It is an assembly of an ion-trap (IT) tandem mass spectrometer with a high-performance liquid chromatograph, an auto sampler with a cooled sample tray and alternative optical diode array detector. The optical detector is compatible with other LC-MS system. Mass spectrometer operates with unit resolution and allows for repeated subsequent fragmentation (MRM)
LC-MS Triple-Quad system
It is an assembly of a triple-quad (QQQ) tandem mass spectrometer with two high-performance liquid chromatographs and auto sampler with a cooled sample tray. It can be connected with an optical detector of the previous system.
Triple quadrupole provides MS / MS spectra and depending on its setting can monitor precursors of the selected fragment, neutral loss etc. Due hyperbolic cross of quadrupole rods the mass spectrometer enables to carry out the analysis using a high resolution mode (H-SRM) and accurate mass measurements with an accuracy of 2 ppm. This accuracy helps determine the molecular formula of unknown substances.
GC-MS Ion-Trap system
It is an assembly of an ion-trap (IT) tandem mass spectrometer with two-channel gas chromatograph with digital control flow of the carrier gas and two injector ports (SSL and PTV). The mass spectrometer is equipped with an electron (EI) and chemical (CI +, Cl) ionization and allows up to five times repeated fragmentation. Due the variable programmable auto sampler allows analysing both liquid and gaseous samples.
HPLC Agilent 1200 with fraction collector
This assembly of a high performance liquid chromatograph allows both the classical HPLC analysis (with UV and/or fluorescent detection) and collect chromatographic fractions. The fractions can be used for further analysis (e.g. mass spectrometry).
Other laboratory equipment for sample preparation
Microbalance Mettler Toledo XP26
Analytical balance Sartorius H110
Rotating vacuum concentrator Christ RVC
Rotating evaporator Büchi
Vacuum SPE manifold
Ultrapure water purification system Millipore RiOs 5 / Milli-Q Synthesis A10
Quantitative determination of specific organic substances in plant and/or other matrix by methods developed on our own.
Examples of analyzes of plant and other matrix:
- Cytokinins including zeatin, isopentenyladenine, benzylaminopurine and their derivatives
- Abscisic acid and indolacetic acid
- Polyamines (diaminopropane, putrescine, cadaverine, spermidine, norspermidine, spermine, thermospermine, norspermine)
- Phenolic acids (gallic, para-hydroxybenzoic, meta-hydroxybenzoic, salicylic, pyrocatechuic, protocatechuic, gentisic, ortho-coumaric, para-coumaric, vanillic, syringic, sinapic, cinnamic, caffeic, ferulic, anisic, chlorogenic)
Development of a method according to the customer requirements and subsequent analysis of supplied samples
Determination of unknown substances
Based on the determination of the accurate mass of ions, isotopic profile, fragmentation spectra and other data, it is possible to determine unknown substances with a certain probability.
- PROCEEDINGS OF THE NAS OF THE USA 106(9) 3609-3614 2009 Fulltext: public PDF link
The role of cytokinins in responses to water deficit in tobacco plants over-expressing trans-zeatin O-glucosyltransferase gene under 35S or SAG12 promotersPLANT, CELL AND ENVIRONMENT 31 341-353 2008 Fulltext: PDF link on intranet only
Evidence for importance of tRNA-dependent cytokinin biosynthetic pathway in the moss Physcomitrella patensJOURNAL OF PLANT GROWTH REGULATION 27 271-281 2008 Fulltext: PDF link on intranet only
Senescence progression in a single darkened cotyledon depends on the light status of the other cotyledon in Cucurbita pepo (zucchini) seedlings: potential involvement of cytokinins and cytokinin oxidase/dehydrogenase activityPHYSIOLOGIA PLANTARUM 134 609-623 2008 Fulltext: PDF link on intranet only
Effects of conditional IPT-Dependent cytokinin overproduction on root architecture of Arabidopsis seedlingsPLANT AND CELL PHYSIOLOGY 49 570-582 2008 Fulltext: PDF link on intranet only
Response of cytokinin pool and cytokinin oxidase/dehydrogenase activity to abscisic acid exhibits organ specificity in peasACTA PHYSIOLOGIAE PLANTARUM 30 151-155 2008
- JOURNAL OF EXPERIMENTAL BOTANY 59 3705-3719 2008
Senescence-induced ectopic expression of A. tumefaciens ipt gene in wheat delays leaf senescence, increases cytokinin content, nitrate influx and nitrate reductase activity but does not affect grain yieldJOURNAL OF EXPERIMENTAL BOTANY 59 (2) 377-387 2008 Fulltext: PDF link on intranet only
1-Aminocyclopropane-1-carboxylic acid and abscisic acid during the germination of sugar beet (Beta vulgaris L.): a comparative study of fruits and seedsJOURNAL OF EXPERIMENTAL BOTANY 58  3047-3060 2007
- BIOTECHNOLOGY AND SUSTAINABLE AGRICULTURE 2006 AND BEYOND 171-174 2007