Laboratory of Mass Spectrometry
Laboratory of Mass Spectrometry – service department
The laboratory was established in 2007 by separation from the larger research division, in which the laboratory team worked as an analytical unit. From the beginning the laboratory was defined as a service centre performing special instrumental analyses and development of new analytical methods. The main task of the laboratory was to provide these services both to other research groups at the home institution and collaborate with other research institutes and universities as well. Currently, we provide analytical services to other entities in the Czech Republic and abroad.
The main part of the laboratory equipment consists of two LC-MS systems, one GC-MS system and one HPLC instrument with fraction collector device.
LC-MS Ion-Trap system
It is an assembly of an ion-trap (IT) tandem mass spectrometer with a high-performance liquid chromatograph, an auto sampler with a cooled sample tray and alternative optical diode array detector. The optical detector is compatible with other LC-MS system. Mass spectrometer operates with unit resolution and allows for repeated subsequent fragmentation (MRM)
LC-MS Triple-Quad system
It is an assembly of a triple-quad (QQQ) tandem mass spectrometer with two high-performance liquid chromatographs and auto sampler with a cooled sample tray. It can be connected with an optical detector of the previous system.
Triple quadrupole provides MS / MS spectra and depending on its setting can monitor precursors of the selected fragment, neutral loss etc. Due hyperbolic cross of quadrupole rods the mass spectrometer enables to carry out the analysis using a high resolution mode (H-SRM) and accurate mass measurements with an accuracy of 2 ppm. This accuracy helps determine the molecular formula of unknown substances.
GC-MS Ion-Trap system
It is an assembly of an ion-trap (IT) tandem mass spectrometer with two-channel gas chromatograph with digital control flow of the carrier gas and two injector ports (SSL and PTV). The mass spectrometer is equipped with an electron (EI) and chemical (CI +, Cl) ionization and allows up to five times repeated fragmentation. Due the variable programmable auto sampler allows analysing both liquid and gaseous samples.
HPLC Agilent 1200 with fraction collector
This assembly of a high performance liquid chromatograph allows both the classical HPLC analysis (with UV and/or fluorescent detection) and collect chromatographic fractions. The fractions can be used for further analysis (e.g. mass spectrometry).
Other laboratory equipment for sample preparation
Microbalance Mettler Toledo XP26
Analytical balance Sartorius H110
Rotating vacuum concentrator Christ RVC
Rotating evaporator Büchi
Vacuum SPE manifold
Ultrapure water purification system Millipore RiOs 5 / Milli-Q Synthesis A10
Quantitative determination of specific organic substances in plant and/or other matrix by methods developed on our own.
Examples of analyzes of plant and other matrix:
- Cytokinins including zeatin, isopentenyladenine, benzylaminopurine and their derivatives
- Abscisic acid and indolacetic acid
- Polyamines (diaminopropane, putrescine, cadaverine, spermidine, norspermidine, spermine, thermospermine, norspermine)
- Phenolic acids (gallic, para-hydroxybenzoic, meta-hydroxybenzoic, salicylic, pyrocatechuic, protocatechuic, gentisic, ortho-coumaric, para-coumaric, vanillic, syringic, sinapic, cinnamic, caffeic, ferulic, anisic, chlorogenic)
Development of a method according to the customer requirements and subsequent analysis of supplied samples
Determination of unknown substances
Based on the determination of the accurate mass of ions, isotopic profile, fragmentation spectra and other data, it is possible to determine unknown substances with a certain probability.
- ACTA PHYSIOLOGIAE PLANTARUM 26 129 2004
Cytokinin N-glucosylation inhibitors suppress deactivation of exogenous cytokinins in radish, but their effect on active endogenous cytokinins is counteracted by other regulatory mechanismsPHYSIOLOGIA PLANTARUM 121  215-222 2004
Changes in endogenous cytokinin levels in cotyledons of Cucurbita pepo (zucchini) during natural and dark-induced senescencePHYSIOLOGIA PLANTARUM 122  133-142 2004
Methyl jasmonate down-regulates endogenous cytokinin levels in cotyledons of Cucurbita pepo (zucchini) seedlingsPHYSIOLOGIA PLANTARUM 122  496-503 2004
Effect of exogenous cytokinins, auxins and adenine on cytokinin N-glucosylation and cytokinin oxidase/dehydrogenase activity in de-rooted radish seedlingsPLANT GROWTH REGULATION 44  15-23 2004
- ACTA PHYSIOLOGIAE PLANTARUM 25  111 2003
- ANNALS OF BOTANY 91  585-597 2003
Cyclin-dependent kinase inhibitor, roscovitine, in combination with exogenous cytokinin, N6-benzyladenine, causes increase of cis-cytokinins in immobilized tobacco cellsBIOTECHNOLOGY LETTERS 25  469-472 2003
O-Glucosylation of cis-Zeatin in Maize. Characterization of Genes, Enzymes, and Endogenous CytokininsPLANT PHYSIOLOGY 131  1374-1380 2003