Plant cell lines in cell morphogenesis research.

Seifertová D., Klíma P., Pařezová M., Petrášek J., Zažímalová E., Opatrný Z.
Methods in Molecular Biology 1080 1080: 215-229, 2014

Abstract: Plant organs and tissues consist of many various cell types, often in different phases of their development. Such complex structures do not allow direct studies on behaviour of individual cells. In contrast, populations of in vitro cultured plant cells represent valuable tool for studying processes on a single cell level, including cell morphogenesis. Here we describe characteristics of well-established model tobacco and Arabidopsis cell lines, and provide detailed protocol on their cultivation, characterization and genetic transformation.
DOI: IEB authors: Petr Klíma, Markéta Fílová, Jan Petrášek, Eva Zažímalová