Plant Reproduction Laboratory
We study a molecular background of flowering in the genus Chenopodium and cytoplasmic male sterility in Silene vulgaris. Both model plants are members of the same order –Caryophyllales. Interest in plant reproduction and plant evolution arose from previous projects focused on the genetic diversity of wild plant species.
The genus Chenopodium contains both short-day and long-day plant species. A short-day species Chenopodium rubrum has been a traditional model of flowering investigated for a long time. A surprising feature of C. rubrum is its sensitivity to photoperiodic induction at seedling stage - at age of about five days. We have identified Chenopodium homologues of the genes CONSTANS and Flowering locus T, which play crucial roles in the course of photoperiodic induction of flowering in Arabidopsis thaliana. We study their expression in seedlings and mature plants of C. rubrum under various physiological conditions.
We noticed that FT- like genes could serve as molecular markers, particularly informative for phylogenetic studies of the complex group C. album sensu lato.
The second project, currently starting in our lab, is an investigation of cytoplasmic male sterility in Silene vulgaris. We focus on variation in mitochondrial transcription among natural populations as well as on the basis of inheritance of mitochondrial DNA. We are searching for the genes encoding cytoplasmic male sterility in this species.
We collaborate with Radomira Vankova who studies metabolism of cytokinins in transgenic tobacco under drought stress. We benefit from methods of plant gene expression analysis, adopted in the course of investigation of flowering (qRT PCR, Northerns with DIG labeled probes).
- Diamanty v prachu. Genetické základy květní indukce u zástupců rodu Chenopodium s kontrastní odpovědí na fotoperiodu. , GA ČR , Helena Štorchová
- Evoluce diploidně-polyploidního komplexu Chenopodium album agg. Společná anebo paralelní evoluce severoamerických a euroasijských druhů? , MŠMT , Helena Štorchová
- Tower of Babel: Mitochondrial-nuclear interactions in the gynodioecious species Silene vulgaris investigated with transcriptomics. , GA ČR , Helena Štorchová
- The role of hybridization and polyploidization in the evolution in Chenopodium album aggregate: From biosystematics to gene expression , GA ČR , Helena Štorchová
- Regulace kvetení u rodu Chenopodium studovaná pomocí transkriptomiky , GA ČR , Helena Štorchová
- Hunters or gardeners? Probing plant-microbe interactions in rootless carnivorous Utricularia from a transcriptomic perspective , GA ČR , Helena Štorchová
- Coexistence of native and inoculated arbuscular mycorrhizal fungi in the roots of host plants , GA ČR , Helena Štorchová
- Dynamic changes of mitochondrial DNA and transcription profiles in Silene vulgaris , GA ČR , Helena Štorchová
- The molecular genetic analysis of the candidate genes responsible for cytoplasmic male sterility in Silene vulgaris , MŠMT , Helena Štorchová
- The identification and studies of expression of the genes involved in photoperiodic flower induction in Chenopodium , GA ČR , Helena Štorchová
Enhanced drought and heat stress tolerance of tobacco plants with ectopically enhanced cytokinin oxidase/dehydrogenase gene expressionJOURNAL OF EXPERIMENTAL BOTANY 64 2805-2815 2013 Fulltext: PDF link on intranet only
Effects of Inoculum Additions in the Presence of a Preestablished Arbuscular Mycorrhizal Fungal CommunityAPPLIED AND ENVIRONMENTAL MICROBIOLOGY 79 6507-6515 2013
- PLoS ONE 7 e30401 2012
Recovery from drought stress in tobacco - an active process associated with the reversal of senescence in some plant parts and the sacrifice of othersPLANT SIGNALLING & BEHAVIOR 7 1-3 2012
Intraradical dynamics of two coexisting isolates of the arbuscular mycorrhizal fungus Glomus intraradices sensu lato as estimated by real-time PCR of mitochondrial DNAAPPLIED AND ENVIRONMENTAL MICROBIOLOGY 78 3630-3637 2012
Intraspecific variation in mitochondrial genome sequence, structure, and gene content in Silene vulgaris, an angiosperm with pervasive cytoplasmic male sterilityNEW PHYTOLOGIST 196 1228-1239 2012
Nanoparticle-specific changes in Arabidopsis thaliana gene expression after exposure to ZnO, TiO2, and fullerene sootJOURNAL OF HAZARDOUS MATERIALS 241– 242 55– 62 2012
- The Flowering Process and its Control in Plants: Gene Expression and Hormone Interactions. 279-287 2011
Tobacco leaves and roots differ in the expression of proline metabolism-related genes in the course of drought stress and subsequent recovery.JOURNAL OF PLANT PHYSIOLOGY 2011
Genome structure and gene expression variation in plant mitochondria, particularly in the genus SileneEvolutionary biology - concepts biodiversity macroevolution and genome evolution 273-289 2011