Production of polyclonal antibodies to the recombinant Potato virus M (PVM) nonstructural triple gene block protein 1 and coat protein

Cerovska N., Moravec T., Plchova H., Hoffmeisterova H., Dedic P.

Keywords: Potato virus M, recombinant protein, coat protein, triple gene block, polyclonal antibodies
Abstract: The genes encoding the coat protein (CP) and triple gene block protein 1 (TGBp1) of Potato virus M (PVM) were cloned into expression vector pET-45b(+) (N-terminal 6xHis tag) and expressed in E. coli Rosetta gami-2(DE3). The purified recombinant antigens were used for polyclonal antibodies raising. The antibodies against recombinant CP were successfully used in Western blot analysis, plate-trapped ELISA and DAS ELISA as a coating for PVM detection in infected potato leaf samples. The antibodies against recombinant nonstructural protein detected the TGBp1 only in Western blot analysis. This is the first report on production of polyclonal antibodies against recombinant coat protein and TGBp1 of PVM and their use for detection of the virus using serological methods.
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IEB authors: Noemi Čeřovská, Hana Hoffmeisterová, Tomáš Moravec, Helena Plchová