Rapid gene isolation using MutChromSeq

Steuernagel, B., Vrána, J., Karafiátová, M., Wulff, B.B.H., Doležel, J.
Wheat rust diseases: Methods and Protocols : 231-243, 2017

Keywords: Chromosome isolation, FISHIS, Flow cytometry and chromosome sorting, Mutational genomics, Gene cloning
Abstract: MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity reduction by flow cytometric chromosome sorting and shotgun sequencing DNA from isolated chromosomes. Comparison of sequences from wild-rype parental chromosome with chromosomes from multiple independently derived mutants identifies causative mutations in a single candidate gene or a noncoding sequence . MutChromSeq does not rely on recombination-based genetic mapping and does not exclude any DNA sequence from being targeted.
DOI: 10.1007/978-1-4939-7249-4
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IEB authors: Jaroslav Doležel, Miroslava Karafiá..., Jan Vrána