Čeština
EnglishTerminal deletions in Hordeum chilense induced by gametocidal activity of chromosome 2C from Aegilops cylindrica
Cifuentes Z., Said M., Cabrera A.
CHROMOSOME RESEARCH 13: 146-149, 2005
Keywords: barley, terminal deletions, gametocidal genes
Abstract: Terminal deletion in Hordeum chilense chromosomes were induced genetically by the gametocidal activity of an alien chromosome 2C derived from Aegilops cylindrica. Diploid organisms such as H. chilense do not tolerate deletions, so the strategy that we followed allowed their obtention in both wheat and tritordeum genetic background. We have crossed fertile tritordeum (H. chilense x T. turgidum amphiploid, 2n = 6x=42, AABBHchHch) with the disomic addition line for chromosome 2C of Ae. cylindrica in wheat conv. ‘Chinese Spring’ (CS). F1 plants (AABBDHch + C) monosomic for 2C were backcrossed to both tritordeum and wheat, respectively, followed by four generations of selfing. The breakpoints of the H. chilense deletions were analyzed by FISH and GISH using both pAs1 and genomic H. chilense DNA as probes. A sample of ten chromosomes from each of the deletions were measured to calculate the approximate fractions length (FL) of the breakpoints from the centromere, in relation to the same intact chromosome arm present in normal tritordeum lines. Terminal deletion were observed in all seven H. chilense chromosomes, except in chromosome 7Hch. Unfortunately, we failed to stablish many of deletions because of sterility. Fertile homozygous plants with deficiencies in the short arm of chromosomes 1Hch and 3Hch, and in the long arm of chromosomes 4Hch and 6Hch were obtained. Deletions in the long arm of chromosome 4Hch were obtained in tritordeum background. Deficiencies in the short arms of both chromosomes 1Hch and 3Hch and in the long arm of chromosome 6Hch were stablished, both as addition or substitution lines, in wheat background. All these plants showed variations in morphological traits.
DOI: IEB authors: Mahmoud Said
CHROMOSOME RESEARCH 13: 146-149, 2005
Keywords: barley, terminal deletions, gametocidal genes
Abstract: Terminal deletion in Hordeum chilense chromosomes were induced genetically by the gametocidal activity of an alien chromosome 2C derived from Aegilops cylindrica. Diploid organisms such as H. chilense do not tolerate deletions, so the strategy that we followed allowed their obtention in both wheat and tritordeum genetic background. We have crossed fertile tritordeum (H. chilense x T. turgidum amphiploid, 2n = 6x=42, AABBHchHch) with the disomic addition line for chromosome 2C of Ae. cylindrica in wheat conv. ‘Chinese Spring’ (CS). F1 plants (AABBDHch + C) monosomic for 2C were backcrossed to both tritordeum and wheat, respectively, followed by four generations of selfing. The breakpoints of the H. chilense deletions were analyzed by FISH and GISH using both pAs1 and genomic H. chilense DNA as probes. A sample of ten chromosomes from each of the deletions were measured to calculate the approximate fractions length (FL) of the breakpoints from the centromere, in relation to the same intact chromosome arm present in normal tritordeum lines. Terminal deletion were observed in all seven H. chilense chromosomes, except in chromosome 7Hch. Unfortunately, we failed to stablish many of deletions because of sterility. Fertile homozygous plants with deficiencies in the short arm of chromosomes 1Hch and 3Hch, and in the long arm of chromosomes 4Hch and 6Hch were obtained. Deletions in the long arm of chromosome 4Hch were obtained in tritordeum background. Deficiencies in the short arms of both chromosomes 1Hch and 3Hch and in the long arm of chromosome 6Hch were stablished, both as addition or substitution lines, in wheat background. All these plants showed variations in morphological traits.
DOI: IEB authors: Mahmoud Said