Inverted laser scanning microscope (2015/2018)
Description	Zeiss LSM880 laser scanning microscope (Axio Observer Z1, inverted) with Definite Focus 2. System is equipped with high resolution Airyscan detector, spectral GaAsP photodetector, two PMT detectors for confocal, one PMT for transmitted light. All scanners are able to performe bidirectional scanning. Capabilities for FCS, RICS, FRAP, FRET and High Dynamic Range (HDR) applications.
Illumination	HXP120 V Illuminator/HAL 100
	Confocal lasers: diode laser (405nm-30), Argon ion laser (458, 488, 514nm), DPSS laser (561nm-10), HeNe laser(633nm)
Objectives	Plan-Apochromat 5x/0.16 M27
	Plan-Apochromat 10x/0.45 DIC M27
	Plan-Apochromat 20x/0.8 DIC M27
	C-Apochromat 10x/0.45 W DIC M27
	C-Apochromat 40x/1.2 W Kor FCS M27
	Plan-Apochromat 63x/1.40 Oil DIC M27
	alpha Plan-Apochromat 100x/1.46 Oil DIC M27
Fluorescence filtercubes	FS49/ DAPI (Ex 365 Em BP 445/50)
	FS38/GFP BP (Ex 470/40 Em BP 525/50)
	FS63 HE/mRFP (Ex BP 572/25 Em BP 629/62)
	Airyscan emission filters:
	BP 420-480 + LP 605 Airyscan
	BP 465-505 + LP 525 Airyscan
	BP 495-550 + LP 570 Airyscan
	BP 555-620 + LP 645 Airyscan
	BP 420-480 + BP 495-550 Airyscan
	BP 420-4445 + BP 465-505 Airyscan
Signal detection	1x high resolution Airyscan detector, 1x spectral high sensitive GaAsP photodetector, 2x standard PMT detectors for confocal; one PMT for transmitted light (T-PMT)
Additional equipment	Definite Focus 2 - focal drift compensation, active anti-vibration table
Software	ZEN black with Experiment Designer, 3D, Tiles, FRAP, FRET, FCS, HDR Analysis modules
Location	building B1 room 008
Phone	ext. 830
Reservation	http://rezervace.ueb.cas.cz

2021
Description	Zeiss LSM900 laser scanning microscope (Axio Observer 7, inverted) with Airyscan 2 with Multiplex mode. Stabilization in z by Definite Focus.3. System is equipped with high resolution Airyscan2 detector, two GaAsP photodetector, one PMT for transmitted light. Capabilities for FRAP, intuitive FRAP, FRET, photomanipulation, macro module, direct processing.
Illumination	Colibri 7 - LED light source (385, 430, 475, 511, 555, 590, 630nm)
	Laser module LM URGB with diode lasers: 405nm (5mW); 488nm (10mW); 561nm (10mW); 640nm 5mW.
Objectives	Plan-Apochromat 10x/0.45 DIC M27
	Plan-Apochromat 20x/0.8 DIC M27
	C-Apochromat 10x/0.45 W DIC M27
	LD LCI Plan-Apo 40x/1,2 Imm Corr DIC M27
	Plan-Apochromat 63x/1.40 Oil DIC M27
	alpha Plan-Apochromat 100x/1.46 Oil DIC M27
Fluorescence filtercubes	FS49/ DAPI (Ex 365 Em BP 445/50)
	FS47/CFP (Ex BP 436/20 Em  BP 480/40)
	FS38/GFP BP (Ex 470/40 Em BP 525/50)
	FS63 HE/mRFP (Ex BP 572/25 Em BP 629/62)
	FS50/CY5 (Ex BP 640/30 Em BP 690/50)
Signal detection	1x high resolution Airyscan2 detector with Multiplex mode (=Fast Airyscan), 2x high sensitive GaAsP photodetector; one PMT for transmitted light (T-PMT)
Additional equipment	Definite Focus 3 - focal drift compensation, active anti-vibration table
Software	ZEN blue with 3D, Tiles&positions, FRAP, Macro, Direct processing modules, APEER connector
Location	building B1, Imaging facility - room 011
Phone	ext. 831
Reservation	http://www.ueb.cas.cz/if

Inverted laser scanning microscope (2018)
Description	Zeiss LSM880 laser scanning microscope in horizontal setup that enables vertical placement of samples suitable for plants. LSM (Axio Observer Z1, inverted) with Definite Focus 2. System is equipped with high resolution Airyscan detector, GaAsP photodetector, two PMT detectors for confocal, one PMT for transmitted light. Capabilities for 3D, Tiles, FRAP and Deconvolution postprocessing in Zen blue.
Illumination	HXP120 V Illuminator/HAL 100
	Confocal lasers: diode laser (405nm-30), Argon ion laser (458, 488, 514nm), DPSS laser (561nm-10), HeNe laser(633nm)
Objectives	Plan-Apochromat 5x/0.16 M27
	Plan-Apochromat 10x/0.45 DIC M27
	Plan-Apochromat 20x/0.8 DIC M27
	LD LCI Plan-Apo 40x/1,2 Imm Corr DIC M27
	Plan-Apochromat 63x/1.40 Oil DIC M27
Fluorescence filtercubes	FS49/ DAPI (Ex 365 Em BP 445/50)
	FS38/GFP BP (Ex 470/40 Em BP 525/50)
	FS63 HE/mRFP (Ex BP 572/25 Em BP 629/62)
	Airyscan emission filters:
	BP 420-480 + LP 605 Airyscan
	BP 465-505 + LP 525 Airyscan
	BP 495-550 + LP 570 Airyscan
	BP 555-620 + LP 645 Airyscan
	BP 420-480 + BP 495-550 Airyscan
	BP 420-4445 + BP 465-505 Airyscan
Signal detection	1x high resolution Airyscan detector, 1x high sensitive GaAsP photodetector, 2x standard PMT detectors for confocal; one PMT for transmitted light (T-PMT)
Additional equipment	Definite Focus 2 - focal drift compensation, active anti-vibration table
	CCD Axiocam 506 mono - as alternative for ocular
Software	ZEN black with 3D, Tiles, FRAP Analysis modules. Zen blue with Deconvolution Licence (theoretical and rael PSF, 4 algorithms)
Location	building B1 room 009
Phone	ext. 474
Reservation	http://rezervace.ueb.cas.cz

2014/upgrade II 2021
Description	Spinning disc (SD) microscope Nikon (Eclipse Ti-E, inverted) with Yokogawa CSU-W1 SD unit (50mm) in dual camera mode. Highly sensitive backilluminated sCMOS cameras Photometrics Prime BSI are used. Microscope is equipped with perfect focus system, Prior motorized stage with trigered z-Piezo. The setup allows simultaneous two-color imaging with two cameras for pairs GFP/RFP, CFP/YFP and single camera sequential recording of signal in xy, z, lambda, time, positions, tiles and any combination of them. NIS SW 5.30 contains advanced tile scan, FRET measurements, Denoise function, deconvolution, jobs.
Illumination	X-cite 120 PC Q Illuminator/HAL 100
	Omicron LightHUB ULTRA: 405, 445, 488, 514, 561, 638nm. Laser power: 120mW - 405nm, other 100mW each laser line.
Objectives	Plan-Apochromat L 4x/0.2
	Plan-Apochromat L 10x/0.45
	Plan-Apochromat L 20x/0.75
	Plan-Apochromat LS 40x/1.25 WI
	Plan-Apochromat L 100x/1.45 Oil
	Plan-Apochromat VC 60x/1.2 W
	Plan-Apochromat L 60x/1.40 Oil - not mounted
Fluorescence filtercubes	Semrock brightline Em 447/60 (DAPI)
	Semrock brightline Em 472/30 (CFP)
	Semrock brightline Em 525/30 (GFP)
	Semrock brightline Em 542/27 (YFP)
	Semrock brightline Em 600/52 (RFP)
	Semrock brightline Em 641/75 (mCherry)
	Semrock brightline Em 708/75 (FarRed)
Signal detection	2x Photometrics Prime BSI, sCMOS Backilluminated, 2048x2048 pixel, QE 95%, 43/63fps, pixel size 6.5 mm
Additional equipment	Perfect Focus System - focal drift compensation; active anti-vibration table
Software	NIS Elements 5.30
Location	building B1 room 009
Phone	ext. 474
Reservation	http://rezervace.ueb.cas.cz

Upright widefield epifluorescence microscope with optical sectioning using structured illumination (2015)
Description	Upright Zeiss AxioImager Z2 Epifluorescence / Widefield Microscope equipped with ApoTome2 module for optical sectionning using structured illumination. Suitable for general-purpose transmitted light and fluorescence widefield microscopy as well as for the imaging with ApoTome2 structured illumination. Fully motorized.
Illumination	HXP120 V Illuminator/VIS LED
Objectives	EC Plan-Neofluar 5x/0.16 M27
	EC Plan-Neofluar 10x/0.3 M27
	EC Plan-Neofluar 20x/0.50 DIC M27
	C-Apochromat 40x/1.2 W Kor DIC M27
	Plan-Apochromat 63x/1.40 Oil DIC M27 (moved to ApoTome2 microscope)
	Plan-Apochromat 100x/1.40 Oil DIC M27
Fluorescence filtercubes	FS01/DAPI (Ex BP365/12 Em LP 397)
	FS47/CFP (Ex BP 436/20 Em  BP 480/40)
	FS09/GFP LP 515nm (Ex  BP 450-490 Em  LP 515)
	FS38/GFP BP (Ex 470/40 Em BP 525/50)
	FS43/CY3, Rhodamine, mOrange  (Ex BP 545/25 Em BP 605/70)
	FS63 HE/mRFP (Ex BP 572/25 Em BP 629/62)
	FS25 TRIPLE band/CFP+GFP+mRFP (Ex TBP 400 + 495 + 570 Em TBP 460 + 530 + 625)
Signal detection	CCD Axiocam 506 mono (6Mpix (2752x2208), 19 fps, pixel size 4.5 mm;  CMOS Axiocam 105 color (5Mpix)
Additional equipment	ApoTome 2; anti-vibration table
Software	Zen pro with Deconvolution Module
Location	building B1 room 008
Phone	ext. 830
Reservation	http://rezervace.ueb.cas.cz

Inverted laser scanning microscope (2006/2020)
Description	Inverted fluorescence widefield microscope Zeiss Axiovert200 equipped with 5Mp colour camera . The setup allows general-purpose transmitted light and fluorescence widefield microscopy in xy, xyz, xyt, xyzt. Suitable for true colour/fluorescence multichannel imaging (e.g. GUS/GFP).
Illumination	X-cite series 120 Illuminator/HAL 100
Objectives	EC Plan-Neofluar 5x/0.15
	EC Plan-Neofluar 10x/0.30 M27
	Plan-Apochromat 20x/0.8 DIC M27
	C-Apochromat 40x/1.2 W Corr FCS DIC M27
	Plan-Neofluar 40x/0.75
	Plan-Apochromat 63x/1.40 Oil DIC M27
Fluorescence filtercubes	FS49 DAPI, Hoechst (Ex 365 Em BP 445/50)
	FS01/DAPI Long Pass (Ex BP365/12 Em LP 397)
	FS38/GFP, FITC BP (Ex 470/40 Em BP 525/50)
	FS20/Rhodamine, TRITC, mOrange (EX BP 546/12 EM BP 575-640)
Signal detection	CMOS camera Axiocam 705 colour: 2464 x 2056 pixels; pixel size 3.45 mm, binning
Additional equipment	Stage holder for multiwell plates. LaCon POC-R-cell cultivation system with peristaltic pump and heating block.
Software	Zen Blue
Location	building B1, 1st floor, room 112
Phone	ext. 474
Reservation	http://rezervace.ueb.cas.cz

2020
Description	Fully motorized fluorescence stereomicroscope with motorized table and imaging system. Many illumination and polarization options: transmitted light: bright field to dark field; Rottermann contrast; LED top illumination: ring light / ring light with diffuser; spot light illumination (SLI) ("gooseneck"). The setup allows light and fluorescence microscopy in xy, xyz, xyt, xyzt. Designed for the detection/screening of fluorescence in larger objects with the possibility of microscopy on closed plates (seedlings, callus, seeds, leafs). Moss microscopy.
Illumination	HXP120 V Illuminator/HAL 100
Objectives	Plan-Apochromat 1.0x
	Plan-Apochromat 0.63x
	Plan-Apochromat 2.0x
Fluorescence filtercubes	M205FA/165FC:
	Filter set DAPI/GFP/mCHerry ET403.5/23; 484/22; 552.5/17x ET450/20; 518/27; 595/43
	Filter set ET DAPI Ex350/50x Em460/50
	Filter set ET CFP ET436/20x ET480/40
	Filter set ET GFP Ex470/40x Em525/50
	Filter set ET CY5 ET620/60x ET700/75
	Filterset ET RFP ET546/10x ET605/70
	Filter set ET mOrange blocks mCherry
	Filter set ET mCherry ET560/40x ET630/75
Signal detection	Leica DMC6200 colour camera 2.2Mp
Additional equipment	Polarization accessories: polarization filters for objective, for ring light and for transmitted light.
Software	LasX Premium with EDF, 3D Visual basic
Location	building B1, room 009
Phone	no
Reservation	http://rezervace.ueb.cas.cz

2019/2021
Description	Imaging system capable of detailed imaging of the seedlings growth dynamics, development of apical hook or phototropism in different light lengths. The core system consists of a one-board raspberry Pi 3B+ computer, IR sensitive 8MP Pi NOIR v2 camera module, and 875nm IR LED backlight illumination. WS2812B RGBWW LEDs serve as a source of side illumination for the phototropic experiments. The whole system is highly customizable - any desirable source of light can be added. Compact size and flexible controls allow to use them in the phytotrons or fridges.
Illumination	HSDL-4220 IR 875 nm LEDs; WS2812B RGBWW LEDs
Signal detection	Raspberry Pi NoIR V2.1 8MP 1080P Camera Module
Additional equipment	Raspberry PI3B+ 1GB RAM, Waveshare 4,3" DSI LCD touch display, IPS, 800×480, I2C, MIPI DSI
Location	Cultivation room 1
Reservation	http://www.ueb.cas.cz/if

2008
Description	Automated pipetting station for in situ hybridizations and immunohistochemical methods. This machine can be used for stainings of both whole mounts and sections on slides. Size of incubation baskets is suitable for Arabidopsis thaliana seedlings and many other materials. Simultaneous incubation of 60samples (objects or slides) is possible with optional cooling or heating of incubation buffers and incubation area. It is possible to recover washing solutions (e.g. antibodies) and use them repeatedly.
Location	building B1, 2nd floor, room 216
Phone	ext 435
Reservation	http://rezervace.ueb.cas.cz

2022/IEB
Description	Windows 11 Pro s instalovaným Windows 10 Pro
	Procesor: Intel® Core i9-12900K
	Paměť: 64 GB DDR5
	Pevný disk: 1 TB M.2 SSD
	Optická mechanika: neobsahuje
	Grafická karta: NVIDIA RTX A4000/16GB
	LAN, WiFi 6E, 1x USB-C, 7x USB 3.2, 3x USB 2.0, 6x DisplayPort, klávesnice, myš
	Záruka: 3 roky (následující pracovní den na místě)
	Monitor: 2x LCD HP U27
Location	building B1, Imaging facility - room 011
Phone	ext. 831
Reservation	http://rezervace.ueb.cas.cz

2012/OPPK
Description	Up to 100 kV TEM with a tungsten filament electron beam source and a 2k x 2k Side-Mounted TEM CCD Camera. Microscope also has a cold trap for liquid nitrogen, that improves vacuum conditions around the specimen area. The microscope is well suited for routine sample screening and the acquisition of micrographs of good quality with a magnification range from 25 - 200.000 x.
Illumination	High Voltage range: 40 to 100 kV in steps of 10 kV, High tension conditioning up to 105 kV, Beam Current: Up to 170 µA, Illumination systém: Two illumination lenses, Condensor 1 and 2
Objectives
	Objective Lens: F = 1.6 mm / Cs = 1.6 mm / Cc = 1.5 mm
	TEM point resolution: 0.45 nm
	TEM line resolution: 0.34 nm
	Minimum probe size: 0.50 µm
	Smallest focus step: 5.0 nm
	Specimen tilt +/- 6°
	User-selectable automatic Intensity Limit to prevent electron beam intensity overload on the specimen. User-selectable INTENSITY ZOOM links the illumination control to the image magnification in order to maintain a constant screen brightness during magnification changes.
Signal detection	The Veleta camera - side-mounted tem camera offering a Peltier-cooled CCD chip with 2k x 2k pixels and a dynamic range of 14 bits.
Software	Morgagni User Interface
Location	building B2, room 016
Phone	ext. 807
Reservation	http://rezervace.ueb.cas.cz

2023/IEB
	Modules: Tracking; Exchange objects; Batch Analysis
	SW Converter Pro
	SW Arivis Virtual Reality
Location	building B1, Imaging facility - room 011
Phone	ext. 831
Reservation	http://rezervace.ueb.cas.cz

Vaheat - temperature control unit for optical microscopes. Made for investigations of temperature-sensitive processes.

LaCon POC-R-cell cultivation system with peristaltic pump and heating block (chamber)

LaCon POC-R-cell cultivation system with peristaltic pump and heating block (heating block)

LaCon POC-R-cell cultivation system with peristaltic pump and heating block (pump)

Ultramicrotome Leica UC7