29.5. - Pawel Kania (NanoTemper Technologies CEE) - When Affinity Matters
When affinity matters.
At NanoTemper Technologies, we are passionate about achieving excellence in everything we do. This has made us the partner of choice for thousands of researchers worldwide in pharmaceutical and biotechnology industries as well as in academic research institutes. The foundation of NanoTemper Technologies is microscale thermophoresis (MST), a powerful technique to quantify biomolecular interactions. MST is a biophysical technique that measures the strength of the interaction between two molecules by detecting variations in fluorescence signal as a result of an IRlaser induced temperature change. The range of the variation in the fluorescence signal correlates with the binding of a ligand to the fluorescent target. MST signal strongly depends on a variety of molecular properties such as size, charge, hydration shell or conformation. Thus, this technique is highly sensitive to virtually any change in molecular properties, allowing for a precise quantification of molecular events independent of the size or nature of the investigated specimen. Along, MST with its unique capabilities enables researchers to work with unpurified proteins, directly in their close to native conditions, such as cell or tissue lysate and membrane fraction as well.