The biochemical characteristics of the physiological activity of beech and spruce embryos

Pešek B., Eliášová K., Vágner M., Vondráková Z.
IUFRO Working Party 2.09.02 : 175 - 179, 2013

Keywords: abscisic acid, indolyl-3-acetic acid, fumarase, somatic embryogenesis, seed quality
Abstract: The endogenous level of ABA can indicate the depth of dormancy and the effect of stratification on beech embryos. ABA can also characterize the ability of somatic embryos to germinate. This accurate method of ABA determination could be successfully used for embryo evaluation. The endogenous level of IAA does not correlate with the dormancy of beech embryos and/or stratification. The endogenous level of IAA increases during polarization of spruce somatic embryos. The increase of the IAA level correlates with the start of somatic embryo germination. The IAA content can characterize the specific steps in the embryo development, but it is not the marker of deep of dormancy. The fumarase activity can clearly distinguish the living and dead cells or tissue. After the rigorous optimization of the method (it is very sensitive to external conditions) it could be a promising method for testing dormancy. The fumarase activity is not a suitable criterion for the evaluation of spruce somatic embryos quality.
IEB authors: Kateřina Eliášová, Bedřich Pešek, Martin Vágner, Zuzana Vondráková