Nie, X., Li, B., Wang, L., Liu, P., Biradar, S.S., Li, T., Doležel, J., Edwards, D., Luo, M., Weining, S.
AMERICAN JOURNAL OF BOTANY : e369–e371, 2012

Keywords: chromosome-arm-specific DNA; flow-sorted chromosomes; next-generation sequencing; Poaceae; Triticum aestivum ; wheat.
Abstract: • Premise of the study: The aim of this study was to assess the feasibility of developing chromosome-arm-specifi c microsatellite markers in wheat on a large scale based on chromosome survey sequences obtained with next-generation sequencing (NGS) technology. • Methods and Results: The Illumina Hi Seq2000 sequencing platform was used to sequence DNA of isolated wheat chromosome- arm 7DL. The data were assembled and microsatellite loci were identifi ed computationally. In total, 16 315 microsatellites were identifi ed from 161 061 assembled contigs. Thirty-three markers were randomly selected for validation across 20 diverse wheat cultivars. Two nulli-tetrasomic stocks were also screened to validate the specifi city of the newly developed markers. • Conclusions: This is the fi rst study on identifi cation of chromosome-arm-specifi c microsatellite markers using NGS technology. These new chromosome-arm-specifi c markers will facilitate saturation of the 7DL genetic map, and their availability will support genetic mapping and positional cloning in wheat.
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IEB authors: Jaroslav Doležel