Essential oil from Myrica rubra leaves inhibits cancer cell proliferation and induces apoptosis in several human intestinal lines

Langhansová L., Hanušová V., Rezek J., Stohanslová B., Ambrož M., Králová V., Vaněk T., Lou J.D., Yun Z.L., Yang J., Skálová L.

Keywords: Myrica rubra, Essential oil, Antiproliferative effect, Cytotoxicity, Apoptosis
Abstract: The leaves of Myrica rubra (Sieb. et Zucc), a subtropical Asian fruit tree with traditional use in folkmedicines, were extracted by hydrodistillation. The antiproliferative effect of obtained M. rubra essen-tial oil (MEO) was tested in human colon and ileocecal adenocarcinoma cell lines HCT8, SW620, SW480,HT29 and Caco2. Cytostatic drug paclitaxel was used as a positive control. MEO significantly inhibitedcell proliferation in concentration-dependant manner in all cell lines. The efficacy of MEO differed inindividual cell lines, with Caco2 being the most sensitive. In these cells, MEO IC50value of 1.5 g/mLwas obtained using xCelligence system for real-time cell monitoring. In cancer cells, MEO induces apo-ptosis and caused significant increase of activities of initiator as well as effector caspases. Contrary tocancer cells, MEO did not affect viability of isolated hepatocytes (as a model of normal non-cancerouscells). GC × GC-TOFMS analysis of MEO chemical composition revealed ˇ-caryophyllene, ˛-humulene,humulene epoxide I, valencene, epi-˛-selinene, -muurolene, ˇ-caryphyllene-oxide, and trans-nerolidolas dominant compounds.
IEB authors: Lenka Langhansová, Jan Rezek, Tomáš Vaněk