Functional divergence of microtubule-associated TPX2 family members in Arabidopsis thaliana

Dvořák Tomaštíková, E., Rutten, T., Dvořák, P., Tugai, A., Ptošková, K., Petrovská, B., van Damme, D., Houben, A., Doležel, J., Demidov, D.

Keywords: Arabidopsis thaliana; aurora kinase 1; in vivo co-localization; kinase assay; phylogeny; targeting protein for Xklp2
Abstract: TPX2 (Targeting Protein for Xklp2) is an evolutionary conserved microtubule-associated protein important for microtubule nucleation and mitotic spindle assembly. The protein was described as an activator of the mitotic kinase Aurora A in humans and the Arabidopsis AURORA1 (AUR1) kinase. In contrast to animal genomes that encode only one TPX2 gene, higher plant genomes encode a family with several TPX2-LIKE gene members (TPXL). TPXL genes of Arabidopsis can be divided into two groups. Group A proteins (TPXL2, 3, 4, and 8) contain Aurora binding and TPX2_importin domains, while group B proteins (TPXL1, 5, 6, and 7) harbor an Xklp2 domain. Canonical TPX2 contains all the above-mentioned domains. We confirmed using in vitro kinase assays that the group A proteins contain a functional Aurora kinase binding domain. Transient expression of Arabidopsis TPX2-like proteins in Nicotiana benthamiana revealed preferential localization to microtubules and nuclei. Co-expression of AUR1 together with TPX2-like proteins changed the localization of AUR1, indicating that these proteins serve as targeting factors for Aurora kinases. Taken together, we visualize the various localizations of the TPX2-LIKE family in Arabidopsis as a proxy to their functional divergence and provide evidence of their role in the targeted regulation of AUR1 kinase activity.
DOI: 10.3390/ijms21062183
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IEB authors: Jaroslav Doležel, Beáta Petrovská, Eva Dvořák Tomašt...