Hormone profiling and the root proteome analysis of itpk1 mutant seedlings of barley (Hordeum vulgare) during the red-light induced photomorphogenesis

Vlčko T., Tarkowská D., Široká J., Pěnčík A., Simerský R., Chamrád I., Lenobel R., Novák O., Ohnoutková L.

Keywords: Inositol phosphate, ITPK, barley, phytohormone, red light
Abstract: Inositol phosphates represent crucial primary metabolites, known as phosphate storage molecules or molecules enabling protein interactions. Inositol phosphates and their pyrophosphate forms direct the assembly of the COP9 signalosome and Cullin Ring Ligase complexes. Thus, inositol phosphates participate in protein degradation, which is a means of signal transduction for several plant hormone classes and a light signaling cascade. In plants, the production of inositol phosphates is maintained by several inositol phosphate kinases, including the group of inositol-1,3,4-trisphosphate 5/6 kinases (ITPKs). The members of ITPKs connect plant phosphate status with light perception and hormonal balance. Photomorphogenesis in red light was investigated using allelic variants of barley Hvitpk1 mutants produced by gene editing. Barley ITPK promoter regions, conserved protein motifs, and expression profiles were investigated to characterize their evolutionary diversification and transcriptional regulation. We focused on ITPK1 relation to auxins, cytokinins, gibberellins, jasmonates, abscisic acid, and salicylic acid in red light-induced photomorphogenesis. Global proteome analyses were conducted in the mutant and wild-type (WT) seedlings grown under continuous red light. Our results demonstrate that different allelic variants of itpk1 mutants manifested defects in producing important phytohormones with consequences for root growth. Mutations in itpk1 affected hormone biosynthesis and metabolite, while proteome analysis revealed significant differences in seedling root proteomes between mutant lines. Based on our results and published data, a model of ITPK activity is proposed depicting them as regulators of hormone signal transduction at the level of protein degradation.
DOI: 10.1016/j.envexpbot.2023.105428 IEB authors: René Lenobel, Ondřej Novák, Ludmila Ohnoutková, Aleš Pěnčík, Jitka Široká, Danuše Tarkowská, Tomáš Vlčko