Čeština
EnglishTransient expression of Human papillomavirus type 16 L2 epitope fused to N- and C-terminus of coat protein of Potato virus X in plants
Cerovska N., Hoffmeisterova H., Moravec T., Plchova H., Folwarczna J., Synkova H., Ryslava H., Ludvikova V., Smahel M.
JOURNAL OF BIOSCIENCES 37: 125-133, 2012
Keywords: Human papillomavirus (HPV-16), L2- and E7-derived epitopes, transient expression
Abstract: Transient expression of foreign genes based on plant viral vectors is a suitable system for the production of relevant immunogens that can be used for the development of a new generation of vaccines against a variety of infectious diseases. In the present study the epitope derived from HPV-16 L2 minor capsid protein (amino acids 108–120) was expressed from Potato virus X (PVX)-based vector pGR106 as N- or C-terminal fusion with the PVX coat protein (PVX CP) in transgenic Nicotiana benthamiana plants. The fusion protein L2108-120-PVX CP was successfully expressed in plants at a level of 170 mg/kg of fresh leaf tissue. The C-terminal fusion protein PVX CP- L2108-120 was expressed using mutated vector sequence to avoid homologous recombination at a level of 8 mg/kg of fresh leaf tissue. Immunogenicity of L2108-120-PVX CP virus-like particles was tested after immunization of mice by subcutaneous injection or tattoo administration. In animal sera the antibodies against the PVX CP and the L2108-120 epitope were found after both methods of vaccine delivery.
DOI:
Fulltext: contact IEB authors
IEB authors: Noemi Čeřovská, Hana Hoffmeisterová, Tomáš Moravec, Helena Plchová, Helena Synková
JOURNAL OF BIOSCIENCES 37: 125-133, 2012
Keywords: Human papillomavirus (HPV-16), L2- and E7-derived epitopes, transient expression
Abstract: Transient expression of foreign genes based on plant viral vectors is a suitable system for the production of relevant immunogens that can be used for the development of a new generation of vaccines against a variety of infectious diseases. In the present study the epitope derived from HPV-16 L2 minor capsid protein (amino acids 108–120) was expressed from Potato virus X (PVX)-based vector pGR106 as N- or C-terminal fusion with the PVX coat protein (PVX CP) in transgenic Nicotiana benthamiana plants. The fusion protein L2108-120-PVX CP was successfully expressed in plants at a level of 170 mg/kg of fresh leaf tissue. The C-terminal fusion protein PVX CP- L2108-120 was expressed using mutated vector sequence to avoid homologous recombination at a level of 8 mg/kg of fresh leaf tissue. Immunogenicity of L2108-120-PVX CP virus-like particles was tested after immunization of mice by subcutaneous injection or tattoo administration. In animal sera the antibodies against the PVX CP and the L2108-120 epitope were found after both methods of vaccine delivery.
DOI:
Fulltext: contact IEB authors
IEB authors: Noemi Čeřovská, Hana Hoffmeisterová, Tomáš Moravec, Helena Plchová, Helena Synková