Transient Gene Expression as a Tool to Monitor and Manipulate the Levels of Acidic Phospholipids in Plant Cells

Noack L.C., Pejchar P., Sekereš J., Jaillais Y., Potocký M.
In: Plant Cell Morphogenesis eds. Cvrčková F. Žárský V. (Humana New York) 1992: 189-199, 2019

Keywords: Microscopy, Nicotiana benthamiana, Nicotiana tabacum, Phosphoinositides, Phospholipid-binding domains, Pollen tube, Transient expression
Abstract: Anionic phospholipids represent only minor fraction of cell membranes lipids but they are critically important for many membrane-related processes, including membrane identity, charge, shape, the generation of second messengers, and the recruitment of peripheral proteins. The main anionic phospholipids of the plasma membrane are phosphoinositides phosphatidylinositol 4-phosphate (PI4P), phosphatidylinositol 4,5-bisphosphate (PI4,5P2), phosphatidylserine (PS), and phosphatidic acid (PA). Recent insights in the understanding of the nature of protein–phospholipid interactions enabled the design of genetically encoded fluorescent molecular probes that can interact with various phospholipids in a specific manner allowing their imaging in live cells. Here, we describe the use of transiently transformed plant cells to study phospholipid-dependent membrane recruitment.
DOI: 10.1007/978-1-4939-9469-4_12 IEB authors: Přemysl Pejchar, Martin Potocký, Juraj Sekereš