Imaging facility of IEB AS CR

Equipment

Inverted laser scanning microscope (2015/2018)
Illumination HXP120 V Illuminator/HAL 100
Confocal lasers: diode laser (405nm-30), Argon ion laser (458, 488, 514nm), DPSS laser (561nm-10), HeNe laser(633nm)
Objectives Plan-Apochromat 5x/0.16 M27
Plan-Apochromat 10x/0.45 DIC M27
Plan-Apochromat 20x/0.8 DIC M27
C-Apochromat 10x/0.45 W DIC M27
C-Apochromat 40x/1.2 W Kor FCS M27
Plan-Apochromat 63x/1.40 Oil DIC M27
alpha Plan-Apochromat 100x/1.46 Oil DIC M27
Fluorescence filtercubes FS49/ DAPI (Ex 365 Em BP 445/50)
FS38/GFP BP (Ex 470/40 Em BP 525/50)
FS63 HE/mRFP (Ex BP 572/25 Em BP 629/62)
Airyscan emission filters:
BP 420-480 + LP 605 Airyscan
BP 465-505 + LP 525 Airyscan
BP 495-550 + LP 570 Airyscan
BP 555-620 + LP 645 Airyscan
BP 420-480 + BP 495-550 Airyscan
BP 420-4445 + BP 465-505 Airyscan
Confocal laser scanning head Zeiss LSM880 laser scanning microscope (Axio Observer Z1, inverted) with Definite Focus 2. System is equipped with high resolution Airyscan detector, spectral GaAsP photodetector, two PMT detectors for confocal, one PMT for transmitted light. All scanners are able to performe bidirectional scanning. Capabilities for FCS, RICS, FRAP, FRET and High Dynamic Range (HDR) applications.
Signal detection 1x high resolution Airyscan detector, 1x spectral high sensitive GaAsP photodetector, 2x standard PMT detectors for confocal; one PMT for transmitted light (T-PMT)
Additional equipment Definite Focus 2 - focal drift compensation, active anti-vibration table
Software ZEN black with Experiment Designer, 3D, Tiles, FRAP, FRET, FCS, HDR Analysis modules
Location building B1 room 008
Phone ext. 830
Reservation http://rezervace.ueb.cas.cz
Inverted laser scanning microscope (2018)
Illumination HXP120 V Illuminator/HAL 100
Confocal lasers: diode laser (405nm-30), Argon ion laser (458, 488, 514nm), DPSS laser (561nm-10), HeNe laser(633nm)
Objectives Plan-Apochromat 5x/0.16 M27
Plan-Apochromat 10x/0.45 DIC M27
Plan-Apochromat 20x/0.8 DIC M27
Plan-Neofluar 40x/0.75
C-Apochromat 40x/1.2 W Kor FCS M27
Plan-Apochromat 63x/1.40 Oil DIC M27
Fluorescence filtercubes FS49/ DAPI (Ex 365 Em BP 445/50)
FS38/GFP BP (Ex 470/40 Em BP 525/50)
FS63 HE/mRFP (Ex BP 572/25 Em BP 629/62)
Airyscan emission filters:
BP 420-480 + LP 605 Airyscan
BP 465-505 + LP 525 Airyscan
BP 495-550 + LP 570 Airyscan
BP 555-620 + LP 645 Airyscan
BP 420-480 + BP 495-550 Airyscan
BP 420-4445 + BP 465-505 Airyscan
Confocal laser scanning head Zeiss LSM880 laser scanning microscope in horizontal setup that enables vertical placement of samples suitable for plants. LSM (Axio Observer Z1, inverted) with Definite Focus 2. System is equipped with high resolution Airyscan detector, GaAsP photodetector, two PMT detectors for confocal, one PMT for transmitted light. Capabilities for 3D, Tiles, FRAP and Deconvolution postprocessing in Zen blue.
Signal detection 1x high resolution Airyscan detector, 1x high sensitive GaAsP photodetector, 2x standard PMT detectors for confocal; one PMT for transmitted light (T-PMT)
Additional equipment Definite Focus 2 - focal drift compensation, active anti-vibration table
CCD Axiocam 506 mono - as alternative for ocular
Software ZEN black with 3D, Tiles, FRAP Analysis modules. Zen blue with Deconvolution Licence (theoretical and rael PSF, 4 algorithms)
Location building B1 room 009
Phone ext. 474
Reservation http://rezervace.ueb.cas.cz
Inverted laser scanning microscope (2006)
Illumination X-cite series 120 Illuminator/HAL 100
Confocal lasers: diode laser (405nm-50); Argon ion laser (458, 477, 488, 514nm); diode laser 488-100 (LIVE); DPSS laser (532nm-75); DPSS laser (561nm-10); HeNe laser(630nm); diode laser 635-35 (LIVE)
Objectives EC Plan-Neofluar 5x/0.15
EC Plan-Neofluar 10x/0.30 M27
Plan-Apochromat 20x/0.8 DIC M27
C-Apochromat 40x/1.2 W Corr FCS DIC M27
Plan-Apochromat 63x/1.40 Oil DIC M27 (moved to ApoTome2 microscope)
LCI Plan-Neofluar 63x/1.3 Im Korr DIC
Fluorescence filtercubes FS49 DAPI, Hoechst (Ex 365 Em BP 445/50)
FS38/GFP, FITC BP (Ex 470/40 Em BP 525/50)
FS20/Rhodamine, TRITC, mOrange (EX BP 546/12 EM BP 575-640)
Confocal laser scanning head Zeiss LSM 5 Duo laser scanning confocal microscope (Axiovert 200, inverted) equipped with fast LIVE 5 line-scanner with one CCD detector and spectral LSM510 point scanner PMT detector and two other single point PMT detectors. The setup allows all applications of point and line laser scanning (x, y, xy, xyz, xyt, xyzt, xyλ) including FRAP, FRET and ratiometric measurements.
Signal detection  1x CCD detector (LIVE), 1x spectral single point PMT detector, 2x single point PMT detectors,  one PMT for transmitted light (T-PMT)
Additional equipment LaCon POC-R-cell cultivation system with peristaltic pump and heating block
Software LSM Image Examiner with  Tiles / Positions, FRAP, FRET modules
Location building B1 room 009
Phone ext. 474
Reservation http://rezervace.ueb.cas.cz
Inverted SD microscope (2014)
Illumination X-cite 120 PC Q Illuminator/HAL 100
Agilent Laser Box MLC400: 405, 488, 561, 640 nm. Laser power 20mW each laser line.  
Objectives Plan-Apochromat L 4x/0.2
Plan-Apochromat L 10x/0.45
Plan-Apochromat L 20x/0.75
Plan-Apochromat LS 40x/1.25 WI
Plan-Apochromat L 100x/1.45 Oil
Plan-Apochromat VC 60x/1.2 W
Plan-Apochromat L 60x/1.40 Oil - not mounted 
Fluorescence filtercubes Quadruple band filter set (390/482/563/640 nm)
Semrock brightline Em 447/60
Semrock brightline Em 525/30
Semrock brightline Em 542/27
Semrock brightline Em 607/36
Semrock brightline Em 685/40
Confocal laser scanning head Spinning disc microscope Nikon (Eclipse Ti-E, inverted)  with Yokogawa CSU-X1 SD unit, high speed filter wheel unit and dual camera system. Microscope is equipped with perfect focus system, Prior motorized stage with z-Piezo, ultra-sensitive EM CCD camera Andor iXon3 897 and Andor Zyla high resolution sCMOS camera. The setup allows sequential or parallel recording of signal in xy, xyt, xyz and xyzt with one or two cameras and FRET measurements.
Signal detection  EM CCD iXON3 897 Andor, 512x512, QE 92%, 34 fps, pixel size 16 mm, Peltier cooling; sCMOS ZYLA Andor, 2560x2160 pixel, QE 57%, 30fps, pixel size 6.5 mm
Additional equipment Perfect Focus System - focal drift compensation; active anti-vibration table
Software NIS Elements 
Location building B1 room 009
Phone ext. 474
Reservation http://rezervace.ueb.cas.cz
Upright widefield epifluorescence microscope with optical sectioning using structured illumination (2015)
Illumination HXP120 V Illuminator/VIS LED
Objectives EC Plan-Neofluar 10x/0.3 M27
EC Plan-Neofluar 20x/0.50 DIC M27
C-Apochromat 40x/1.2 W Kor DIC M27
Plan-Apochromat 63x/1.40 Oil DIC M27 (moved to ApoTome2 microscope)
Plan-Apochromat 100x/1.40 Oil DIC M27
Fluorescence filtercubes FS01/DAPI (Ex BP365/12 Em LP 397)
FS47/CFP (Ex BP 436/20 Em  BP 480/40)
FS09/GFP LP 515nm (Ex  BP 450-490 Em  LP 515)
FS38/GFP BP (Ex 470/40 Em BP 525/50)
FS43/CY3, Rhodamine, mOrange  (Ex BP 545/25 Em BP 605/70)
FS63 HE/mRFP (Ex BP 572/25 Em BP 629/62)
FS25 TRIPLE band/CFP+GFP+mRFP (Ex TBP 400 + 495 + 570 Em TBP 460 + 530 + 625)
Confocal laser scanning head Upright Zeiss AxioImager Z2 Epifluorescence / Widefield Microscope equipped with ApoTome2 module for optical sectionning using structured illumination. Suitable for general-purpose transmitted light and fluorescence widefield microscopy as well as for the imaging with ApoTome2 structured illumination. Fully motorized.  
Signal detection   CCD Axiocam 506 mono (6Mpix (2752x2208), 19 fps, pixel size 4.5 mm;  CMOS Axiocam 105 color (5Mpix)
Additional equipment ApoTome 2; anti-vibration table
Software Zen pro with Deconvolution Module
Location building B1 room 008
Phone ext. 830
Reservation http://rezervace.ueb.cas.cz
2012
Location building B2 basement room
Reservation http://rezervace.ueb.cas.cz
2008
Confocal laser scanning head Automated pipetting station for in situ hybridizations and immunohistochemical methods. This machine can be used for stainings of both whole mounts and sections on slides. Size of incubation baskets is suitable for Arabidopsis thaliana seedlings and many other materials. Simultaneous incubation of 60samples (objects or slides) is possible with optional cooling or heating of incubation buffers and incubation area. It is possible to recover washing solutions (e.g. antibodies) and use them repeatedly.
Location building B1, 2nd floor, room 216
Phone ext 435
Reservation http://rezervace.ueb.cas.cz

LaCon POC-R-cell cultivation system with peristaltic pump and heating block (chamber)

LaCon POC-R-cell cultivation system with peristaltic pump and heating block (heating block)

LaCon POC-R-cell cultivation system with peristaltic pump and heating block (pump)