Imaging facility of IEB AS CR

Equipment

Inverted laser scanning microscope (2015/2018)
Illumination HXP120 V Illuminator/HAL 100
Confocal lasers: diode laser (405nm-30), Argon ion laser (458, 488, 514nm), DPSS laser (561nm-10), HeNe laser(633nm)
Objectives Plan-Apochromat 5x/0.16 M27
Plan-Apochromat 10x/0.45 DIC M27
Plan-Apochromat 20x/0.8 DIC M27
C-Apochromat 10x/0.45 W DIC M27
C-Apochromat 40x/1.2 W Kor FCS M27
Plan-Apochromat 63x/1.40 Oil DIC M27
alpha Plan-Apochromat 100x/1.46 Oil DIC M27
Fluorescence filtercubes FS49/ DAPI (Ex 365 Em BP 445/50)
FS38/GFP BP (Ex 470/40 Em BP 525/50)
FS63 HE/mRFP (Ex BP 572/25 Em BP 629/62)
Airyscan emission filters:
BP 420-480 + LP 605 Airyscan
BP 465-505 + LP 525 Airyscan
BP 495-550 + LP 570 Airyscan
BP 555-620 + LP 645 Airyscan
BP 420-480 + BP 495-550 Airyscan
BP 420-4445 + BP 465-505 Airyscan
Confocal laser scanning head Zeiss LSM880 laser scanning microscope (Axio Observer Z1, inverted) with Definite Focus 2. System is equipped with high resolution Airyscan detector, spectral GaAsP photodetector, two PMT detectors for confocal, one PMT for transmitted light. All scanners are able to performe bidirectional scanning. Capabilities for FCS, RICS, FRAP, FRET and High Dynamic Range (HDR) applications.
Signal detection 1x high resolution Airyscan detector, 1x spectral high sensitive GaAsP photodetector, 2x standard PMT detectors for confocal; one PMT for transmitted light (T-PMT)
Additional equipment Definite Focus 2 - focal drift compensation, active anti-vibration table
Software ZEN black with Experiment Designer, 3D, Tiles, FRAP, FRET, FCS, HDR Analysis modules
Location building B1 room 008
Phone ext. 830
Reservation http://rezervace.ueb.cas.cz
Inverted laser scanning microscope (2018)
Illumination HXP120 V Illuminator/HAL 100
Confocal lasers: diode laser (405nm-30), Argon ion laser (458, 488, 514nm), DPSS laser (561nm-10), HeNe laser(633nm)
Objectives Plan-Apochromat 5x/0.16 M27
Plan-Apochromat 10x/0.45 DIC M27
Plan-Apochromat 20x/0.8 DIC M27
LD LCI Plan-Apo 40x/1,2 Imm Corr DIC M27
Plan-Apochromat 63x/1.40 Oil DIC M27
Fluorescence filtercubes FS49/ DAPI (Ex 365 Em BP 445/50)
FS38/GFP BP (Ex 470/40 Em BP 525/50)
FS63 HE/mRFP (Ex BP 572/25 Em BP 629/62)
Airyscan emission filters:
BP 420-480 + LP 605 Airyscan
BP 465-505 + LP 525 Airyscan
BP 495-550 + LP 570 Airyscan
BP 555-620 + LP 645 Airyscan
BP 420-480 + BP 495-550 Airyscan
BP 420-4445 + BP 465-505 Airyscan
Confocal laser scanning head Zeiss LSM880 laser scanning microscope in horizontal setup that enables vertical placement of samples suitable for plants. LSM (Axio Observer Z1, inverted) with Definite Focus 2. System is equipped with high resolution Airyscan detector, GaAsP photodetector, two PMT detectors for confocal, one PMT for transmitted light. Capabilities for 3D, Tiles, FRAP and Deconvolution postprocessing in Zen blue.
Signal detection 1x high resolution Airyscan detector, 1x high sensitive GaAsP photodetector, 2x standard PMT detectors for confocal; one PMT for transmitted light (T-PMT)
Additional equipment Definite Focus 2 - focal drift compensation, active anti-vibration table
CCD Axiocam 506 mono - as alternative for ocular
Software ZEN black with 3D, Tiles, FRAP Analysis modules. Zen blue with Deconvolution Licence (theoretical and rael PSF, 4 algorithms)
Location building B1 room 009
Phone ext. 474
Reservation http://rezervace.ueb.cas.cz
Inverted SD microscope (2014/upgrade 2019)
Illumination X-cite 120 PC Q Illuminator/HAL 100
Agilent Laser Box MLC400: 405, 488, 561, 640 nm. Laser power 20mW each laser line.  
Objectives Plan-Apochromat L 4x/0.2
Plan-Apochromat L 10x/0.45
Plan-Apochromat L 20x/0.75
Plan-Apochromat LS 40x/1.25 WI
Plan-Apochromat L 100x/1.45 Oil
Plan-Apochromat VC 60x/1.2 W
Plan-Apochromat L 60x/1.40 Oil - not mounted 
Fluorescence filtercubes Quadruple band filter set (390/482/563/640 nm)
Semrock brightline Em 447/60
Semrock brightline Em 525/30
Semrock brightline Em 542/27
Semrock brightline Em 607/36
Semrock brightline Em 685/40
Confocal laser scanning head Spinning disc microscope Nikon (Eclipse Ti-E, inverted)  with Yokogawa CSU-X1 SD unit, high speed filter wheel unit and dual camera system. Microscope is equipped with perfect focus system, Prior motorized stage with z-Piezo, highly sensitive sCMOS Backilluminated Photometrics Prime cameras (BSI and 95B). The setup allows sequential or parallel recording of signal in xy, xyt, xyz and xyzt with one or two cameras and FRET measurements.
Signal detection  Photometrics Prime 95B, sCMOS Backilluminated, 1200x1200, QE 95%, 41/82 fps, pixel size 11 mm; Photometrics Prime BSI, sCMOS Backilluminated, 2048x2048 pixel, QE 95%, 43/63fps, pixel size 6.5 mm
Additional equipment Perfect Focus System - focal drift compensation; active anti-vibration table
Software NIS Elements 
Location building B1 room 009
Phone ext. 474
Reservation http://rezervace.ueb.cas.cz
Upright widefield epifluorescence microscope with optical sectioning using structured illumination (2015)
Illumination HXP120 V Illuminator/VIS LED
Objectives EC Plan-Neofluar 5x/0.16 M27
EC Plan-Neofluar 10x/0.3 M27
EC Plan-Neofluar 20x/0.50 DIC M27
C-Apochromat 40x/1.2 W Kor DIC M27
Plan-Apochromat 63x/1.40 Oil DIC M27 (moved to ApoTome2 microscope)
Plan-Apochromat 100x/1.40 Oil DIC M27
Fluorescence filtercubes FS01/DAPI (Ex BP365/12 Em LP 397)
FS47/CFP (Ex BP 436/20 Em  BP 480/40)
FS09/GFP LP 515nm (Ex  BP 450-490 Em  LP 515)
FS38/GFP BP (Ex 470/40 Em BP 525/50)
FS43/CY3, Rhodamine, mOrange  (Ex BP 545/25 Em BP 605/70)
FS63 HE/mRFP (Ex BP 572/25 Em BP 629/62)
FS25 TRIPLE band/CFP+GFP+mRFP (Ex TBP 400 + 495 + 570 Em TBP 460 + 530 + 625)
Confocal laser scanning head Upright Zeiss AxioImager Z2 Epifluorescence / Widefield Microscope equipped with ApoTome2 module for optical sectionning using structured illumination. Suitable for general-purpose transmitted light and fluorescence widefield microscopy as well as for the imaging with ApoTome2 structured illumination. Fully motorized.  
Signal detection   CCD Axiocam 506 mono (6Mpix (2752x2208), 19 fps, pixel size 4.5 mm;  CMOS Axiocam 105 color (5Mpix)
Additional equipment ApoTome 2; anti-vibration table
Software Zen pro with Deconvolution Module
Location building B1 room 008
Phone ext. 830
Reservation http://rezervace.ueb.cas.cz
Inverted laser scanning microscope (2006/2020)
Illumination X-cite series 120 Illuminator/HAL 100
Objectives EC Plan-Neofluar 5x/0.15
EC Plan-Neofluar 10x/0.30 M27
Plan-Apochromat 20x/0.8 DIC M27
C-Apochromat 40x/1.2 W Corr FCS DIC M27
Plan-Neofluar 40x/0.75
Plan-Apochromat 63x/1.40 Oil DIC M27
Fluorescence filtercubes FS49 DAPI, Hoechst (Ex 365 Em BP 445/50)
FS01/DAPI Long Pass (Ex BP365/12 Em LP 397)
FS38/GFP, FITC BP (Ex 470/40 Em BP 525/50)
FS20/Rhodamine, TRITC, mOrange (EX BP 546/12 EM BP 575-640)
Confocal laser scanning head Inverted fluorescence widefield microscope Zeiss Axiovert200 equipped with 5Mp colour camera . The setup allows general-purpose transmitted light and fluorescence widefield microscopy in xy, xyz, xyt, xyzt. Suitable for true colour/fluorescence multichannel imaging (e.g. GUS/GFP). 
Signal detection  CMOS camera Axiocam 705 colour: 2464 x 2056 pixels; pixel size 3.45 mm, binning
Additional equipment Stage holder for multiwell plates. LaCon POC-R-cell cultivation system with peristaltic pump and heating block. 
Software Zen Blue
Location building B1, 1st floor, room 112
Phone ext. 474
Reservation http://rezervace.ueb.cas.cz
2020
Illumination HXP120 V Illuminator/HAL 100
Objectives Plan-Apochromat 1.0x
Plan-Apochromat 0.63x
Plan-Apochromat 2.0x
Fluorescence filtercubes M205FA/165FC:
Filter set DAPI/GFP/mCHerry ET403.5/23; 484/22; 552.5/17x ET450/20; 518/27; 595/43
Filter set ET DAPI Ex350/50x Em460/50
Filter set ET CFP ET436/20x ET480/40
Filter set ET GFP Ex470/40x Em525/50
Filter set ET CY5 ET620/60x ET700/75
Filterset ET RFP ET546/10x ET605/70
Filter set ET mOrange blocks mCherry
Filter set ET mCherry ET560/40x ET630/75
Confocal laser scanning head Fully motorized fluorescence stereomicroscope with motorized table and imaging system. Many illumination and polarization options: transmitted light: bright field to dark field; Rottermann contrast; LED top illumination: ring light / ring light with diffuser; spot light illumination (SLI) ("gooseneck"). The setup allows light and fluorescence microscopy in xy, xyz, xyt, xyzt. Designed for the detection/screening of fluorescence in larger objects with the possibility of microscopy on closed plates (seedlings, callus, seeds, leafs). Moss microscopy.
Signal detection   Leica DMC6200 colour camera 2.2Mp
Additional equipment Polarization accessories: polarization filters for objective, for ring light and for transmitted light.
Software LasX Premium with EDF, 3D Visual basic
Location building B1, room 010
Phone no
Reservation http://rezervace.ueb.cas.cz
2012
Location building B2 basement room
Reservation http://rezervace.ueb.cas.cz
2008
Confocal laser scanning head Automated pipetting station for in situ hybridizations and immunohistochemical methods. This machine can be used for stainings of both whole mounts and sections on slides. Size of incubation baskets is suitable for Arabidopsis thaliana seedlings and many other materials. Simultaneous incubation of 60samples (objects or slides) is possible with optional cooling or heating of incubation buffers and incubation area. It is possible to recover washing solutions (e.g. antibodies) and use them repeatedly.
Location building B1, 2nd floor, room 216
Phone ext 435
Reservation http://rezervace.ueb.cas.cz

LaCon POC-R-cell cultivation system with peristaltic pump and heating block (chamber)

LaCon POC-R-cell cultivation system with peristaltic pump and heating block (heating block)

LaCon POC-R-cell cultivation system with peristaltic pump and heating block (pump)