Čeština
EnglishThe influence of Potato virus Y infection on the ultrastructure of Pssu-ipt transgenic tobacco
Schnablová, Renáta; Synková, Helena; Čeřovská, Noemi
INTERNATIONAL JOURNAL OF PLANT SCIENCES 166 [5]: 713-721, 2005
Keywords: ultrastructure; viral infection; Potato virus Y-NTN
Abstract: We studied the effect of Potato virus Y-NTN (PVY) on the cell ultrastructure of control ( rooted control plants [ C] and control plants grafted onto control rootstock [C/C]) and transgenic Pssu-ipt plants overproducing cytokinins (CK; rooted [T] or grafted [T/C]). The PVY infection caused visible symptoms, i.e., reduction of leaf surface and plant growth, acceleration of leaf senescence, veinal necrosis, and leaf distortion in all infected C plants; in T and C/C plants, plant age affected whether symptoms of infection occurred. Even though DAS-ELISA proved the presence of virus coat protein in all studied plants, the symptoms of infection were never observed in T/C plants. Relative content of virus proteins in cells correlated with the stage of symptom development in infected plants. A massive accumulation of virus proteins was found in the cytoplasm of infected plants, and it was proved by immunocytochemical methods. The most prominent effect of viral infection was a decrease of volume density of starch, an increase of volume density of plastoglobuli in chloroplasts, and more abundant cores inside peroxisomes in C plants. Although virus particles were not found inside chloroplasts, they formed large aggregates adjacent to cell organelles - nuclei, chloroplasts, mitochondria, and peroxisomes. Both transgenic plants and C/C plants were less sensitive to viral infection. We concluded that not only CK overproduction but also the cultivation method of plants may influence the sensitivity of plants to biotic stress.
DOI:
IEB authors: Noemi Čeřovská, Helena Synková
INTERNATIONAL JOURNAL OF PLANT SCIENCES 166 [5]: 713-721, 2005
Keywords: ultrastructure; viral infection; Potato virus Y-NTN
Abstract: We studied the effect of Potato virus Y-NTN (PVY) on the cell ultrastructure of control ( rooted control plants [ C] and control plants grafted onto control rootstock [C/C]) and transgenic Pssu-ipt plants overproducing cytokinins (CK; rooted [T] or grafted [T/C]). The PVY infection caused visible symptoms, i.e., reduction of leaf surface and plant growth, acceleration of leaf senescence, veinal necrosis, and leaf distortion in all infected C plants; in T and C/C plants, plant age affected whether symptoms of infection occurred. Even though DAS-ELISA proved the presence of virus coat protein in all studied plants, the symptoms of infection were never observed in T/C plants. Relative content of virus proteins in cells correlated with the stage of symptom development in infected plants. A massive accumulation of virus proteins was found in the cytoplasm of infected plants, and it was proved by immunocytochemical methods. The most prominent effect of viral infection was a decrease of volume density of starch, an increase of volume density of plastoglobuli in chloroplasts, and more abundant cores inside peroxisomes in C plants. Although virus particles were not found inside chloroplasts, they formed large aggregates adjacent to cell organelles - nuclei, chloroplasts, mitochondria, and peroxisomes. Both transgenic plants and C/C plants were less sensitive to viral infection. We concluded that not only CK overproduction but also the cultivation method of plants may influence the sensitivity of plants to biotic stress.
DOI: