Shifting the limits in wheat research and breeding using a fully annotated reference genome

International Wheat Genome Sequencing Consortium (IWGSC); IWGSC RefSeq principal investigators: Appels, R., Eversole, K., Feuillet, C., Keller, B., Rogers, J., Stein, N.; IWGSC whole-genome assembly principal investigators: Pozniak, C.J., Stein, N., Choulet, F., Distelfeld, A., Eversole, K., Poland, J., Rogers, J., Ronen, G., Sharpe, A.G.; Whole-genome sequencing and assembly: Pozniak, C., Ronen, G., Stein, N., Barad, O., Baruch, K., Choulet, F., Keeble-Gagnère, G., Mascher, M., Sharpe, A.G., Ben-Zvi, G., Josselin, A.A.; Hi-C data-based scaffolding: Stein, N., Mascher, M., Himmelbach, A.; Whole-genome assembly quality control and analyses: Choulet, F., Keeble-Gagnère, G., Mascher, M., Rogers, J., Balfourier, F., Gutierrez-Gonzalez, J., Hayden, M., Josselin, A.A., Koh, C., Muehlbauer, G., Pasam, R.K., Paux, E., Pozniak, C.J., Rigault, P., Sharpe, A.G., Tibbits, J., Tiwari, V.; Pseudomolecule assembly: Choulet, F., Keeble-Gagnère, G., Mascher, M., Josselin, A.A., Rogers, J.; RefSeq genome structure and gene analyses: Spannagl, M., Choulet, F., Lang, D., Gundlach, H., Haberer, G., Keeble-Gagnère, G., Mayer, K.F.X., Ormanbekova, D., Paux, E., Prade, V., Šimková, H., Wicker, T.; Automated annotation: Choulet, F., Spannagl, M., Swarbreck, D., Rimbert, H., Felder, M., Guilhot, N., Gundlach, H., Haberer, G., Kaithakottil, G., Keilwagen, J., Lang, D., Leroy, P., Lux, T., Mayer, K.F.X., Twardziok, S., Venturini, L.; Manual gene curation: Appels, R., Rimbert, H., Choulet, F., Juhász, A., Keeble-Gagnère, G.; Subgenome comparative analyses: Choulet, F., Spannagl, M., Lang, D., Abrouk, M., Haberer, G., Keeble-Gagnère, G., Mayer, K.F.X., Wicker, T.; Transposable elements: Choulet, F., Wicker, T., Gundlach, H., Lang, D., Spannagl, M.; Phylogenomic analyses: Lang, D., Spannagl, M., Appels, R., Fischer, I.; Transcriptome analyses and RNA-seq data: Uauy, C., Borrill, P., Ramirez-Gonzalez, R.H., Appels, R., Arnaud, D., Chalabi, S., Chalhoub, B., Choulet, F., Cory, A., Datla, R., Davey, M.W., Hayden, M., Jacobs, J., Lang, D., Robinson, S.J., Spannagl, M., Steuernagel, B., Tibbits, J., Tiwari, V., van Ex, F., Wulff, B.B.H.; Whole-genome methylome: Pozniak, C.J., Robinson, S.J., Sharpe, A.G., Cory, A.; Histone mark analyses: Benhamed, M., Paux, E., Bendahmane, A., Concia, L., Latrasse, D.; BAC chromosome MTP IWGSC–Bayer Whole-Genome Profiling (WGP) tags: Rogers, J., Jacobs, J., Alaux, M., Appels, R., Bartoš, J., Bellec, A., Berges, H., Doležel, J., Feuillet, C., Frenkel, Z., Gill, B., Korol, A., Letellier, T., Olsen, O.A., Šimková, H., Singh, K., Valárik, M., van der Vossen, E., Vautrin, S., Weining, S.; Chromosome LTC mapping and physical mapping quality control: Korol, A., Frenkel, Z., Fahima, T., Glikson, V., Raats, D., Rogers, J.; RH mapping: Tiwari, V., Gill, B., Paux, E., Poland, J.; Optical mapping: Doležel, J., Číhalíková, J., Šimková, H., Toegelová, H., Vrána, J.; Recombination analyses: Sourdille, P., Darrier, B.; Gene family analyses: Appels, R., Spannagl, M., Lang, D., Fischer, I., Ormanbekova, D., Prade, V.; CBF gene family: Barabaschi, D., Cattivelli, L.; Dehydrin gene family: Hernandez, P., Galvez, S., Budak, H.; NLR gene family: Steuernagel, B., Jones, J.D.G., Witek, K., Wulff, B.B.H., Yu, G.; PPR gene family: Small, I., Melonek, J., Zhou, R.; Prolamin gene family: Juhász, A., Belova, T., Appels, R., Olsen, O.A.; WAK gene family: Kanyuka, K., King, R.; Stem solidness (SSt1) QTL team: Nilsen, K., Walkowiak, S., Pozniak, C.J., Cuthbert, R., Datla, R., Knox, R., Wiebe, K., Xiang, D.; Flowering locus C (FLC) gene team: Rohde, A., Golds, T.; Genome size analysis: Doležel, J., Čížková, J., Tibbits, J.; MicroRNA and tRNA annotation: Budak, H., Akpinar, B.A., Biyiklioglu, S.; Genetic maps and mapping: Muehlbauer, G., Poland, J., Gao, L., Gutierrez-Gonzalez, J., N'Daiye, A.; BAC libraries and chromosome sorting: Doležel, J., Šimková, H., Číhalíková, J., Kubaláková, M., Šafář, J., Vrána, J.; BAC pooling, BAC library repository, and access: Berges, H., Bellec, A., Vautrin, S.; IWGSC sequence and data repository and access: Alaux, M., Alfama, F., Adam-Blondon, A.F., Flores, R., Guerche, C., Letellier, T., Loaec, M., Quesneville, H.; Physical maps and BAC-based sequences: 1A BAC sequencing and assembly: Pozniak, C.J., Sharpe, A.G., Walkowiak, S., Budak, H., Condie, J., Ens, J., Koh, C., Maclachlan, R., Tan, Y., Wicker, T.; 1B BAC sequencing and assembly: Choulet, F., Paux, E., Alberti, A., Aury, J.M., Balfourier, F., Barbe, V., Couloux, A., Cruaud, C., Labadie, K., Mangenot, S., Wincker, P.; 1D, 4D, and 6D physical mapping: Gill, B., Kaur, G., Luo, M., Sehgal, S.; 2AL physical mapping: Singh, K., Chhuneja, P., Gupta, O.P., Jindal, S., Kaur, P., Malik, P., Sharma, P., Yadav, B.; 2AS physical mapping: Singh, N.K., Khurana, J., Chaudhary, C., Khurana, P., Kumar, V., Mahato, A., Mathur, S., Sevanthi, A., Sharma, N., Tomar, R.S.; 2B, 2D, 4B, 5BL, and 5DL IWGSC–Bayer Whole-Genome Profiling (WGP) physical maps: Rogers, J., Jacobs, J., Alaux, M., Bellec, A., Berges, H., Doležel, J., Feuillet, C., Frenkel, Z., Gill, B., Korol, A., van der Vossen, E., Vautrin, S.; 3AL physical mapping: Gill, B., Kaur, G., Luo, M., Sehgal, S.; 3DS physical mapping and BAC sequencing and assembly: Bartoš, J., Holušová, K., Plíhal, O.; 3DL BAC sequencing and assembly: Clark, M.D., Heavens, D., Kettleborough, G., Wright, J.; 4A physical mapping, BAC sequencing, assembly, and annotation: Valárik, M., Abrouk, M., Balcárková, B., Holušová, K., Hu, Y., Luo, M.; 5BS BAC sequencing and assembly: Salina, E., Ravin, N., Skryabin, K., Beletsky, A., Kadnikov, V., Mardanov, A., Nesterov, M., Rakitin, A., Sergeeva, E.; 6B BAC sequencing and assembly: Handa, H., Kanamori, H., Katagiri, S., Kobayashi, F., Nasuda, S., Tanaka, T., Wu, J.; 7A physical mapping and BAC sequencing: Appels, R., Hayden, M., Keeble-Gagnère, G., Rigault, P., Tibbits, J.; 7B physical mapping, BAC sequencing, and assembly: Olsen, O.A., Belova, T., Cattonaro, F., Jiumeng, M., Kugler, K., Mayer, K.F.X., Pfeifer, M., Sandve, S., Xun, X., Zhan, B.; 7DS BAC sequencing and assembly: Šimková, H., Abrouk, M., Batley, J., Bayer, P.E., Edwards, D., Hayashi, S., Toegelová, H., Tulpová, Z., Visendi, P.; 7DL physical mapping and BAC sequencing: Weining, S., Cui, L., Du, X., Feng, K., Nie, X., Tong, W., Wang, L.; Figures: Borrill, P., Gundlach, H., Galvez, S., Kaithakottil, G., Lang, D., Lux, T., Mascher, M., Ormanbekova, D., Prade, V., Ramirez-Gonzalez, R.H., Spannagl, M., Stein, N., Uauy, C., Venturini, L.; Manuscript writing team: Stein, N., Appels, R., Eversole, K., Rogers, J., Borrill, P., Cattivelli, L., Choulet, F., Hernandez, P., Kanyuka, K., Lang, D., Mascher, M., Nilsen, K., Paux, E., Pozniak, C.J., Ramirez-Gonzalez, R.H., Šimková, H., Small, I., Spannagl, M., Swarbreck, D., Uauy, C.
SCIENCE 361: 7191, 2018

Keywords:
Abstract: Wheat (TriticumaestivumL.) is the most widely cultivated crop on Earth, contributing about a fifth of the total calories consumed by humans. Consequently, wheat yields and production affect the global economy, and failed harvests can lead to social unrest. Breeders continuously strive to develop improved varieties by fine-tuning genetically complex yield and end-use quality parameters while maintaining stable yields and adapting the crop to regionally specific biotic and abiotic stresses.
DOI: 10.1126/ science.aar7191
Fulltext: contact IEB authors
IEB authors: Jan Bartoš, Jana Čížková, Jaroslav Doležel, Kateřina Holušová, Jan Šafář, Hana Šimková, Helena Toegelová ..., Zuzana Tulpová, Miroslav Valárik, Jan Vrána