Centre of Plant Structural and Functional Genomics
The Centre of Plant Structural and Functional Genomics has been established to study the structure and function of nuclear genome in higher plants. The main focus is on economically important crops like cereals, legumes, bananas and plantains.
Methodological approaches involve classical and molecular cytogenetics, flow cytometry and sorting, recombinant DNA techniques and construction of chromosome and chromosome-arm specific BAC libraries. Because of the complexity of research projects, the Laboratory collaborates with many research groups throughout the world. The laboratory staff is also involved in teaching and technology transfer, including consultancy services in the above mentioned areas.
Flow cytogenetics involve chromosome analysis and sorting using flow cytometry applications of sorted chromosomes include physical mapping using PCR, targeted isolation of molecular markers, isolation of low-copy (genic) sequences from specific genome regions, high-throughput physical mapping of DNA sequences by hybridization to DNA arrays, as well as HAPPY and optical mapping.
Chromosome-based BAC libraries recources
The most attractive use of sorted chromosomes is the production of chromosome- and chromosome arm-specific DNA libraries. Such libraries provide a permanent and easily accessible molecular resource specific for a particular part of the genome, is expected to have a major impact on wheat genomics.
Flow cytometry is powerful technique with many application. We used it for genome size estimation, ploidy measurement and flow karyotype analysis of higher plants.
We interested in molecular evolution and organization of Musa genome and serve as the Musa Genome Resources Centre (MGRC). The primary aim of the Centre is to support research activities of the Musa Genomics Consortium by distributing Musa genome resources to Consortium Members and by developing new resources.
Study on Festuca-Lolium complex
We are using molecular cytogenetic methods (FISH and GISH) to study genomic constitution of Festuca × Lolium hybrids, chromosome pairing during meiosis, and to map BAC clones on chromosomes of Festuca pratensis. In collaboration with other labs, we also developed set of introgression lines of all F. pratensis chromosomes in tetraploid L. multiflorum and DArT chip for five species within Festuca-Lolium complex.
head of the laboratorysenior scientist
- Mgr. Jan Bartoš Ph.D.
deputy head of the laboratorysenior scientist
- Mgr. Aleš Pečinka Ph.D.
- Ing. Radoslava Kvasnič...
graduated technical assistant
- Ing. Michaela Holecová
- Mgr. Denisa Beránková Ph.D.
- Mgr. Alžběta Doležalová PhD.
- Mgr. Jaroslav Fiľo
- Mgr. Lucie Hloušková
- Mgr. Jakub Juračka
- Mgr. Kateřina Kaduchová
- Mgr. Zuzana Korchanová
- Mgr. Martin Kovačik
- Mgr. Adam Lampar
- Mgr. Šimon Pavlů
- Mgr. Beáta Strejčková
- Mgr. Zuzana Tulpová Ph.D
- Mgr. Petr Urbiš
- M.Sc. Fen Yang
- Mgr. Jana Zwyrtková Ph.D.
- Eliška Čamková DiS.
- Mgr. Eva Janáková
Identification of a major QTL-controlling resistance to the subtropical race 4 of Fusarium oxysporum f. sp. cubense in Musa acuminata ssp. malaccensisPATHOGENS 12 289 2023 Fulltext: PDF link on intranet only
Both male and female meiosis contribute to non-Mendelian inheritance of parental chromosomes in interspecific plant hybrids (Lolium X Festuca)NEW PHYTOLOGIST 238 624-636 2023 Fulltext: PDF link on intranet only
- NATURE 615 652-659 2023 Fulltext: PDF link on intranet only
- NUCLEIC ACIDS RESEARCH 51 2641-2654 2023 Fulltext: PDF link on intranet only
- EUCARPIA CEREALS SECTION CONFERENCE MAY 15-20.2023 CEREAL RESEARCH NONPROFIT LTD. (GK) SZEGED HUNGARY 40-40 2023
Characterization of Aegilops biuncialis introgressions in a wheat prebreeding population using DArTseq genotypingEUCARPIA CEREALS SECTION CONFERENCE MAY 15-20.2023 CEREAL RESEARCH NONPROFIT LTD. (GK) SZEGED HUNGARY 32-32 2023
- NATURE GENETICS 55 914-920 2023 Fulltext: PDF link on intranet only
- NATURE GENETICS 55 921-926 2023 Fulltext: PDF link on intranet only
- Plant cytogenetics and cytogenomics: Methods and Protocols 2672 2023 Fulltext: PDF link on intranet only
- Plant cytogenetics and cytogenomics: Methods and Protocols 2672 177-200 2023 Fulltext: PDF link on intranet only