Centre of Plant Structural and Functional Genomics
Research Topics
The Centre of Plant Structural and Functional Genomics has been established to study the structure and function of nuclear genome in higher plants. The main focus is on economically important crops like cereals, legumes, bananas and plantains.
Methodological approaches involve classical and molecular cytogenetics, flow cytometry and sorting, recombinant DNA techniques and construction of chromosome and chromosome-arm specific BAC libraries. Because of the complexity of research projects, the Laboratory collaborates with many research groups throughout the world. The laboratory staff is also involved in teaching and technology transfer, including consultancy services in the above mentioned areas.
Flow cytogenetics
Flow cytogenetics involve chromosome analysis and sorting using flow cytometry applications of sorted chromosomes include physical mapping using PCR, targeted isolation of molecular markers, isolation of low-copy (genic) sequences from specific genome regions, high-throughput physical mapping of DNA sequences by hybridization to DNA arrays, as well as HAPPY and optical mapping.
Chromosome-based BAC libraries recources
The most attractive use of sorted chromosomes is the production of chromosome- and chromosome arm-specific DNA libraries. Such libraries provide a permanent and easily accessible molecular resource specific for a particular part of the genome, is expected to have a major impact on wheat genomics.
Flow cytometry
Flow cytometry is powerful technique with many application. We used it for genome size estimation, ploidy measurement and flow karyotype analysis of higher plants.
Banana studies
We interested in molecular evolution and organization of Musa genome and serve as the Musa Genome Resources Centre (MGRC). The primary aim of the Centre is to support research activities of the Musa Genomics Consortium by distributing Musa genome resources to Consortium Members and by developing new resources.
Study on Festuca-Lolium complex
We are using molecular cytogenetic methods (FISH and GISH) to study genomic constitution of Festuca × Lolium hybrids, chromosome pairing during meiosis, and to map BAC clones on chromosomes of Festuca pratensis. In collaboration with other labs, we also developed set of introgression lines of all F. pratensis chromosomes in tetraploid L. multiflorum and DArT chip for five species within Festuca-Lolium complex.
Workers
- Mgr. Zuzana Antalová Ph.D.
- Ing. Václav Bačovský PhD.
- Naděžda Čadová
- Bc. Marie Chudecká
- prof. RNDr. Ivo Frébort CSc., Ph.D.
- Mgr. Simona Martikánová
- PhD Serhii Mykhailyk
- MSc. Mehrdad Shahbazi
head of the laboratorysenior scientist
- Mgr. Jan Bartoš Ph.D.
deputy head of the laboratorysenior scientist
- Mgr. Aleš Pečinka Ph.D.
project manager
- Ing. Radoslava Kvasnič...
senior scientist
- Mgr. Eva Hřibová Ph.D.
- RNDr. David Kopecky Ph.D.
- RNDr. Jan Šafář Ph.D.
- Ing. Hana Šimková CSc.
- Mgr. Miroslav Valárik Ph.D.
scientist
- Nicolas Blavet Ph.D.
- Ing. Radim Čegan Ph.D.
- Prof. Ing. Jaroslav Doležel DrSc.
- Mgr. Pavla Navratilova PhD
- Mgr. Anna Nowicka Ph.D.
scientific assistant
- Mgr. Iva Ilíková PhD.
- M.Sc. Mahmoud Said Ph.D.
graduated technical assistant
- Mgr. Lucie Bílková
- Mgr. Gabriela Majzlíková
- ing. Petr Navratil
- Mgr. Tereza Šlajsová
- Mgr. Magdaléna Stejska...
THS employee
- Ing. Michaela Holecová
pracovník THS
- ing. Petra Neplechová DiS.
- Ing. Lucie Pavlovičová
postdoc
- Mgr. Jana Čížková Ph.D.
- Mgr. Kateřina Holušová Ph.D.
- Mgr. Miroslava Karafiá... Ph.D.
- Marek Szecówka PhD
- Mgr. Jana Szecówka (Kn... PhD.
- Mgr. Helena Toegelová ... Ph.D.
- Mgr. Eva Dvořák Tomašt... Ph.D.
- M.Sc. Jovanka Vladejić
postgraduate student
- Mgr. Denisa Beránková Ph.D.
- Mgr. Alžběta Doležalová PhD.
- Mgr. Lucie Hloušková
- Mgr. Jakub Juračka
- Mgr. Kateřina Kaduchová
- Mgr. Zuzana Korchanová
- Mgr. Martin Kovačik
- Mgr. Adam Lampar
- Mgr. Šimon Pavlů
- Mgr. Beáta Strejčková
- Mgr. Zuzana Tulpová Ph.D
- Mgr. Petr Urbiš
- M.Sc. Fen Yang
- Mgr. Jana Zwyrtková Ph.D.
assistant
- Eliška Čamková DiS.
technician
maintenance
gatekeeper
maternity leave
- Mgr. Eva Janáková Ph.D.
Research projects
Publications
2024
- SCIENTIFIC REPORTS 14 3119 2024 Fulltext: PDF link on intranet only
- THEORETICAL AND APPLIED GENETICS 137 48 2024 Fulltext: PDF link on intranet only
- The wheat genome; compendium of plant genomes 27-50 2024 Fulltext: PDF link on intranet only
- NATURE COMMUNICATIONS 15 503 2024 Fulltext: PDF link on intranet only
- MOLECULAR BREEDING 44 7 2024 Fulltext: PDF link on intranet only
- NATURE 628 804-810 2024 Fulltext: PDF link on intranet only
- FRONTIERS IN PLANT SCIENCE 15 1396553 2024 Fulltext: PDF link on intranet only
- PLANT JOURNAL 118 1102-1118 2024 Fulltext: PDF link on intranet only
- SCIENTIFIC REPORTS 14 12998 2024 Fulltext: PDF link on intranet only
- NEW PHYTOLOGIST 243 1840-1854 2024 Fulltext: PDF link on intranet only