Isotope Laboratory

Group head: 
Zdeněk Wimmer
Vídeňská 1083, 142 20 Praha 4
wimmeratbiomed [dot] cas [dot] cz
+420 241 062 381
+420 241 062 150

Fields of work:

  • Synthesis of cytokinins, including labeling with 3H in specific carbon centers
  • Custom synthesis of compounds labeled with 3H and/or 14C
  • Synthesis and investigation of urea derivatives with activity on suppressing plant stress
  • Synthesis and investigation of derivatives of purine, pyrimidine and other heterocycles with cytotoxic and anti-tumor activity
  • Investigation of root system of selected plants in water purification
  • Analysis and investigation of effect of heavy metals and/or metaloids on plant hyperaccumulators


  • Zemanová V., Pavlíková D., Dobrev P.I., Motyka V., Pavlík M.*: Endogenous phytohormone profiles in Pteris fern species differing in arsenic accumulating ability. Environmental and Experimental Botany 2019, 166, Article Number: 103822. (DOI: 10.1016/j.envexpbot.2019.103822).
  • Valuskova, P.; Riljak, V.; Forczek, S.T.; Farar, V.; Myslivecek, J.: Variability in the Drug Response of M-4 Muscarinic Receptor Knockout Mice During Day and Night Time. Frontiers in Pharmacology 2019 10, Article Number 237, (DOI: 10.3389/fphar.2019.00237).
  • Jansa, J.; Forczek, S.T.; Rozmos, M.; Puschel, D.; Bukovska, P.; Hrselova, H. (2019) Arbuscular mycorrhiza and soil organic nitrogen: network of players and interactions. Chemical and Biological Technologies in Agriculture 6, Article Number: UNSP 10 (DOI: 10.1186/s40538-019-0147-2).


  • Popov M., Zemanová V., Kotrba P., Pavlík M., Pavlíková D.: Arsenic accumulation and speciation in Pteris cretica and Pteris straminea. FEBS Open Bio 2018, 8(S1), 164-165.
  • Valušková, P., Forczek, S. T., Farár, V., Mysliveček, J.: The deletion of M4 muscarinic receptors increases motor activity in females in the dark phase, Brain Behav 2018; e01057, (DOI: 10.1002/brb3.1057).
  • Valušková, P., Farár, V., Forczek, S. T., Křížová, I., Mysliveček, J.: Autoradiography of 3H-pirenzepine and 3H-AFDX-384 in Mouse Brain Regions: Possible Insights into M1, M2, and M4 Muscarinic Receptors Distribution, Front Pharmacol 2018, 9:124 (DOI: 10.3389/fphar.2018.00124)


  • Zemanová V.*, Pavlík M., Pavlíková D.* (2017): Cadmium toxicity induced contrasting patterns of concentrations of free sarcosine, specific amino acids and selected microelements in two Noccaea species. PLoS One 2017, 12(5), Article Number: e0177963. (DOI: 10.1371/journal.pone.0177963).
  • Pavlíková D., Zemanová V., Pavlík M.*: The contents of free amino acids and elements in As-hyperaccumulator Pteris cretica and non-hyperaccumulator Pteris straminea during reversible senescence. Plant Soil and Environment 2017, 63(10), 455-460. (DOI: 10.17221/606/2017-PSE).
  • Pavlík M.*, Zemanová V., Pavlíková D., Kyjaková P., Hlavsa T.: Regulation of odd-numbered fatty acid content plays an important part in the metabolism of the hyperaccumulator Noccaea spp. adapted to oxidative stress. Journal of Plant Physiology 2017, 208, 94-101. (DOI: 10.1016/j.jplph.2016.09.014).


  • Forczek S. T., Pavlík M., Holík J., Rederer L., Ferenčík M.: The natural chlorine cycle - Formation of the carcinogenic and greenhouse gas compound chloroform in drinking water reservoirs. Chemosphere 2016, 157, 190-199. (DOI: 10.1016/j.chemosphere.2016.05.017).
  • Zemanová V., Pavlík M., Pavlíková D., Hnilička F., Vondráčková S.: Responses to Cd stress in two Noccaea species (Noccaea praecox and Noccaea caerulescens) originating from two contaminated sites in Mežica, Slovenia and Redlschlag, Austria. Archives of Environmental Contamination and Toxicology 2016, 70(3), 464-474. (DOI: 10.1007/s00244-015-0198-8).
  • Procházková D., Pavlíková D., Pavlík M.: Chapter 5. Sulphur: role in alleviation of environmental stress in crop plants. 84-96. In: Azooz M. M., Ahmad P., (Eds.): Plant-Environment Interaction. Responses and Approaches to Mitigate Stress. Willey Blackwell, 2016, 350 pp. (ISBN: 978-1-119-08099-2).
  • Břendová, K., Zemanová V., Pavlíková D., Tlustoš P.: Utilization of biochar and activated carbon to reduce Cd, Pb and Zn phytoavailability and phytotoxicity for plants. Journal of Environmental Management 2016, 181, 637-645. (DOI: 10.1016/j.jenvman.2016.06.042).


  • Zemanová V., Pavlík M., Kyjaková P., Pavlíková D. (2015): Fatty acid profiles of ecotypes of hyperaccumulator Noccaea caerulescens growing under cadmium stress (2015, 180, 27). Journal of Plant Physiology 2015, 183, 84-84. (DOI: 10.1016/j.jplph.2015.06.002); Paper correction.
  • Zemanová V., Pavlík M., Kyjaková P., Pavlíková D.: Fatty acid profiles of ecotypes of hyperaccumulator Noccaea caerulescens growing under cadmium stress. Journal of Plant Physiology 2015, 180, 27-34. (DOI: 10.1016/j.jplph.2015.02.012).
  • Zemanová V., Pavlík M., Pavlíková D., Kyjaková P.: Changes in the contents of amino acids and the profile of fatty acids in response to cadmium contamination in spinach. Plant Soil and Environment 2015, 61(6), 285-290. (DOI: 10.17221/274/2015-PSE).

Synthesis and investigation of derivatives of plant sterols and triterpenoid acids with better bioavailability, cytotoxicity, antimicrobial activity and/or supramolecular characteristics.
Shared research:
University of Chemistry and Technology in Prague (
Professor Erkki T. Kolehmainen, University of Jyväskylä, Jyväskylä, Finland (
Professor Nonappa, Aalto University, Espoo, Finland (
Professor Juyoung Yoon, Ewha Womans University, Seoul, Republic of Korea (
Dr. David Šaman, Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences (
Prof. Miroslav Strnad, Palacký University, Olomouc, Czech Republic (
Prof. Miroslav Šlouf, Institute of Macromolecular Chemistry, Czech Academy of Sciences (
Dr. Marie Sajfrtová, Institute of Chemical Process Fundamentals, Czech Academy of Sciences (

  • Bildziukevich U., Malík M., Özdemir Z., Rárová L., Janovská L., Šlouf M., Šaman D., Šarek J., Nonappa, Wimmer Z.: Spermine amides of selected triterpenoid acids: Dynamic supramolecular systems formation influences cytotoxicity of the drugs. J. Mater. Chem. B, (DOI: 10.1039/C9TB01957J).
  • Bildziukevich U., Özdemir Z., Wimmer Z.: Recent achievements in medicinal and supramolecular chemistry of betulinic acid and its derivatives. Molecules 2019, 24, 3546.
  • Bildziukevich U., Rárová L., Janovská L., Šaman D., Wimmer Z.: Enhancing effect of cystamine in its amides with betulinic acid as antimicrobial and antitumor agent in vitro. Steroids 2019, 148, 91-98.
  • Tatikonda R., Bulatov E., Özdemir Z., Nonappa, Haukka M.: Infinite coordination polymer networks: metallogelation of aminopyridine conjugates and in situ silver nanoparticle formation. Soft Matter 2019, 15, 442-451.


  • Özdemir Z., Bildziukevich U., Wimmerová M., Macůrková A., Lovecká P., Wimmer Z.: Plant adaptogens: Natural Medicaments for 21st century? ChemistrySelect 2018, 3, 2196-2214.
  • Bildziukevich U., Rárová L., Šaman D., Wimmer Z.: Picolyl amides of betulinic acid as antitumor agents causing tumor cell apoptosis. Eur. J. Med. Chem. 2018, 145, 41-50.


  • Özdemir Z., Bildziukevich U., Šaman D., Havlíček L., Rárová L., Navrátilová L., Wimmer Z.: Amphiphilic derivatives of (3β,17β)-3-hydroxyandrost-5-ene-17-carboxylic acid. Steroids 2017, 128, 58-67.
  • Škorpilová L., Rimpelová S., Jurášek M., Buděšínský M., Lokajová J., Effenberg R., Slepička P., Ruml T., Kmoníčková E., Drašar P.B., Wimmer Z.: BODIPY-based fluorescent liposomes with seskviterpene lactone trilobolide. Beilstein J. Org. Chem. 2017, 13, 1316-1324.
  • Wimmerová M., Siglerová V., Šaman D., Šlouf M., Kaletová E., Wimmer Z.: Improved enzyme-mediated synthesis and supramolecular self-assembly of naturally occurring conjugates of β-sitosterol. Steroids 2017, 117, 38-43.


  • Sperduto C., Smolková M., Jurášek M., Malachowska M., Venanzi M., Monti D., Mancini G., Wimmer Z., Drašar P.: Steroidal ribbons from (3α,5β,20S)-3-hydroxy-20-methyl-pregan-21-oic acid. Lett. Org. Chem. 2016, 13, 711-717.
  • Malachowska M., Sperduto C., Darmostuk M., Monti D., Venanzi M. Mancini G., D’Acunto C.W., Králová J., Ruml T., Wimmer Z., Drašar P.B.: Porphyrins with directly meso-attached disaccharide moieties: Synthesis, self-assembly and cellular study. J. Porphyrin. Phthalocyanin. 2016, 20, 773-784.


  • Bildziukevich U., Vida N., Rárová L., Kolář M., Šaman D., Havlíček L., Drašar P., Wimmer Z.: Polyamine derivatives of betulinic acid and β-sitosterol: A comparative investigation. Steroids 2015, 100, 27-35.
  • Forczek S.T., Laturnus F., Doležalová J., Holík J., Wimmer Z.: Emission of climate relevant volatile organochlorines by plants occurring in temperate forests. Plant Soil Environ. 2015, 61, 103-108.
  • Lapčík O., Wimmer Z., Opletal L., Moravcová J., Čopíková J., Drašar P.: Natural Taste-Sour Substances. Chem. Listy 2015, 109, 488-491.

head of the laboratory
senior scientist

senior scientist


scientific assistant

graduated technical assistant


postgraduate student



Number of publications: 175