Centre of Plant Structural and Functional Genomics

Contacts
Group head: 
Jan Bartoš
Centre: 
Olomouc
Address: 
Šlechtitelů 31, 783 71, Olomouc - Holice, Česká republika
Email: 
bartosatueb [dot] cas [dot] cz
Phone: 
+420 585 238 711
www: 
Centre of Plant Structural and Functional Genomics

Research Topics

The Centre of Plant Structural and Functional Genomics has been established to study the structure and function of nuclear genome in higher plants. The main focus is on economically important crops like cereals, legumes, bananas and plantains.

Methodological approaches involve classical and molecular cytogenetics, flow cytometry and sorting, recombinant DNA techniques and construction of chromosome and chromosome-arm specific BAC libraries. Because of the complexity of research projects, the Laboratory collaborates with many research groups throughout the world. The laboratory staff is also involved in teaching and technology transfer, including consultancy services in the above mentioned areas.

Flow cytogenetics

Flow cytogenetics involve chromosome analysis and sorting using flow cytometry applications of sorted chromosomes include physical mapping using PCR, targeted isolation of molecular markers, isolation of low-copy (genic) sequences from specific genome regions, high-throughput physical mapping of DNA sequences by hybridization to DNA arrays, as well as HAPPY and optical mapping.

Chromosome-based BAC libraries recources

The most attractive use of sorted chromosomes is the production of chromosome- and chromosome arm-specific DNA libraries. Such libraries provide a permanent and easily accessible molecular resource specific for a particular part of the genome, is expected to have a major impact on wheat genomics.

Flow cytometry

Flow cytometry is powerful technique with many application. We used it for genome size estimation, ploidy measurement and flow karyotype analysis of higher plants.

Banana studies

We interested in molecular evolution and organization of Musa genome and serve as the Musa Genome Resources Centre (MGRC). The primary aim of the Centre is to support research activities of the Musa Genomics Consortium by distributing Musa genome resources to Consortium Members and by developing new resources.

Study on Festuca-Lolium complex

We are using molecular cytogenetic methods (FISH and GISH) to study genomic constitution of Festuca × Lolium hybrids, chromosome pairing during meiosis, and to map BAC clones on chromosomes of Festuca pratensis. In collaboration with other labs, we also developed set of introgression lines of all F. pratensis chromosomes in tetraploid L. multiflorum and DArT chip for five species within Festuca-Lolium complex.

Workers: 

head of the laboratory
senior scientist

deputy head of the laboratory
senior scientist

project manager

senior scientist

scientist

scientific assistant

graduated technical assistant

THS employee

pracovník THS

postdoc

postgraduate student

assistant

technician

maintenance

gatekeeper

maternity leave

Research projects: 
Publications: 
Number of publications: 578

2014

  • Flow cytometric chromosome sorting from diploid progenitors of bread wheat, T. urartu, Ae. speltoides and Ae. tauschii
    Molnár, I., Kubaláková, M., Šimková, H., Farkas, A., Cseh, A., Megyeri, M., Vrána, J., Molnár-Láng, M., Doležel, J.
    THEORETICAL AND APPLIED GENETICS 127 1091-1104 2014 Fulltext: PDF link on intranet only
  • A chromosome-based draft sequence of the hexaploid bread wheat (Triticum aestivum) genome
    Mayer, K.F.X., Rogers, J., Doležel, J., Pozniak, C., Eversole, K., Feuillet, C., Gill, B., Friebe, B., Lukaszewski, A.J., Sourdille, P., Endo, T.R., Kubaláková, M., Číhalíková, J., Dubská, Z., Vrána, J., Šperková, R., Šimková, H., Febrer, M., Clissold, L., McLay, K., Singh, K., Chhuneja, P., Singh, N.K., Khurana, J., Akhunov, E., Choulet, F., Alberti, A., Barbe, V., Wincker, P., Kanamori, H., Kobayashi, F., Itoh, T., Matsumoto, T., Sakai, H., Tanaka, T., Wu, J.Z., Ogihara, Y., Handa, H., Maclachlan, P.R., Sharpe, A., Klassen, D., Edwards, D., Batley, J., Olsen, O.A., Sandve, S.R., Lien, S., Steuernagel, B., Wulff, B., Caccamo, M., Ayling, S., Ramirez-Gonzalez, R.H., Clavijo, B.J., Wright, J., Pfeifer, M., Spannagl, M., Martis, M.M., Mascher, M., Chapman, J., Poland, J.A., Scholz, U., Barry, K., Waugh, R., Rokhsar, D.S., Muehlbauer, G.J., Stein, N., Gundlach, H., Zytnicki, M., Jamilloux, V., Quesneville, H., Wicker, T., Faccioli, P., Colaiacovo, M., Stanca, A.M., Budak, H., Cattivelli, L., Glover, N., Pingault, L., Paux, E., Sharma, S., Appels, R., Bellgard, M., Chapman, B., Nussbaumer, T., Bader, K.C., Rimbert, H., Wang, S.C., Knox, R., Kilian, A., Alaux, M., Alfama, F., Couderc, L., Guilhot, N., Viseux, C., Loaec, M., Keller, B., Praud, S.
    SCIENCE 345 1-11 2014 Fulltext: PDF link on intranet only
  • Karyotype analysis in Agropyron cristatum
    Said M., Cabrera A., Doležel J.
    Cereals for Food Feed and Fuel Challenge for Global Improvement Joint EUCARPIA Cereal Section and ITMI Conference. June 29 - July 4 | Wernigerode-Germany 178-178 2014 Fulltext: PDF link on intranet only
  • Genome-wide analysis of repeat diversity across the family Musaceae
    Novák, P., Hřibová, E., Neumann, P., Koblížková, A., Doležel, J., Macas, J.
    PLOS ONE 9(6) e98918 2014 Fulltext: PDF link on intranet only
  • Structural and functional partitioning of bread wheat chromosome 3B
    Choulet, F., Alberti, A., Theil, S., Glover, N., Barbe, V., Daron, J., Pingault, L., Sourdille, P., Couloux, A., Paux, E., Leroy, P., Mangenot, S., Guilhot, N., Le Gouis, J., Balfourier, F., Alaux, M., Jamilloux, V., Poulain, J., Durand, C., Bellec, A., Gaspin, C., Šafář, J., Doležel, J., Rogers, J., Vandepoele, K., Aury, JM., Mayer, K., Berges, H., Quesneville, H., Wincker, P., Feuillet, C.
    SCIENCE 345(6194) 1249721 2014 Fulltext: PDF link on intranet only
  • Common wheat chromosome 5B composition analysis using low-coverage 454 sequencing
    Sergeeva, E.M., Afonnikov, D.A., Koltunova, M.K., Gusev, V.D., Miroshnichenko, L.A., Vrána, J., Kubaláková, M., Poncet, C., Sourdille, P., Feuillet, C., Doležel, J., Salina, E.A.
    THE PLANT GENOME 7(2) 1-16 2014 Fulltext: PDF link on intranet only
  • Multiple, concentration-dependent effects of sucrose, auxins and cytokinins in explant cultures of kale and tobacco
    Luštinec, J., Cvrčková, F., Čížková, J., Doležel, J., Kamínek, M., Žárský, V.
    ACTA PHYSIOLOGIAE PLANTARUM 36 1981-1991 2014 Fulltext: PDF link on intranet only
  • Proteomic analysis of barley cell nuclei purified by flow sorting
    Petrovská, B., Jeřábková, H., Chamrád, I., Vrána, J., Lenobel, R., Uřinovská, J., Sebela, M., Doležel, J.
    CYTOGENETIC AND GENOME RESEARCH 143 78-86 2014 Fulltext: PDF link on intranet only
  • A chromosomal genomics approach to assess and validate the desi and kabuli draft chickpea genome assemblies
    Ruperao, P., Chan, C.K., Azam, S., Karafiátová, M., Hayashi, S., Cížková, J., Saxena, R.K., Šimková, H., Song, C., Vrána, J., Chitikineni, A., Visendi, P., Gaur, P.M., Millán, T., Singh, K.B., Taran, B., Wang, J., Batley, J., Doležel, J., Varshney, R.K., Edwards, D.
    PLANT BIOTECHNOLOGY JOURNAL 12 778-786 2014 Fulltext: PDF link on intranet only
  • Molecular and Flow Cytometric Karyotype analysis in Agropyron cristatum
    Said M., Cabrera A., Vrána J., Číhalíková J., Doležel J.
    Plant Molecular Cytogenetics in Genomic and Postgenomic Era Conference. 23-24 September | Katowice-Poland 91-91 2014 Fulltext: PDF link on intranet only
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