Arabidopsis non-specific phospholipase C1: characterization and its involvement in response to heat stress

Krčková Z., Brouzdová J., Daněk M., Kocourková D., Rainteau D., Ruelland E., Valentová O., Pejchar P., Martinec J.

Klíčová slova: non-specific phospholipase C, heat stress, Arabidopsis thaliana, phospholipids, diacylglycerol
Abstrakt: The Arabidopsis non-specific phospholipase C1 (NPC) protein family is encoded by the genes NPC1 – NPC6. It has been shown that NPC4 and NPC5 possess phospholipase C activity; NPC3 has lysophosphatidic acid phosphatase activity. NPC3, 4 and 5 play roles in the responses to hormones and abiotic stresses. NPC1, 2 and 6 has not been studied functionally yet. We found that Arabidopsis NPC1 expressed in Escherichia coli possesses phospholipase C activity in vitro. This protein was able to hydrolyse phosphatidylcholine to diacylglycerol. NPC1-green fluorescent protein was localized to secretory pathway compartments in Arabidopsis roots. In the knock out T-DNA insertion line NPC1 (npc1) basal thermotolerance was impaired compared with wild-type (WT); npc1 exhibited significant decreases in survival rate and chlorophyll content at the seventh day after heat stress (HS). Conversely, plants overexpressing NPC1 (NPC1-OE) were more resistant to HS compared with WT. These findings suggest that NPC1 is involved in the plant response to heat.
DOI: Autoři z ÚEB: Jitka Brouzdová, Michal Daněk, Daniela Kocourková, Zuzana Krčková, Jan Martinec, Přemysl Pejchar