Čeština
EnglishKaryotype differentiation in cultivated chickpea revealed by oligopainting fluorescence in situ hybridization
Doležalová, A., Sládeková, L., Šimoníková, D., Holušová, K, Karafiátová, M., Varshney, R.K., Doležel, J., Hřibová, E.
FRONTIERS IN PLANT SCIENCE 12: 791303, 2022
Klíčová slova: Cicer arietinum L., kabuli type, desi type, oligopainting FISH, chromosome identification, chromosome translocation
Abstrakt: Chickpea (Cicer arietinum L.) is one of the main sources of plant proteins in the Indian subcontinent and West Asia, where two different morphotypes, desi and kabuli, are grown. Despite the progress in genome mapping and sequencing, the knowledge of the chickpea genome at the chromosomal level, including the longrange molecular chromosome organization, is limited. Earlier cytogenetic studies in chickpea suffered from a limited number of cytogenetic landmarks and did not permit to identify individual chromosomes in the metaphase spreads or to anchor pseudomolecules to chromosomes in situ. In this study, we developed a system for fast molecular karyotyping for both morphotypes of cultivated chickpea. We demonstrate that even draft genome sequences are adequate to develop oligo-fluorescence in situ hybridization (FISH) barcodes for the identification of chromosomes and comparative analysis among closely related chickpea genotypes. Our results show the potential of oligo-FISH barcoding for the identification of structural changes in chromosomes, which accompanied genome diversification among chickpea cultivars. Moreover, oligo- FISH barcoding in chickpea pointed out some problematic, most probably wrongly assembled regions of the pseudomolecules of both kabuli and desi reference genomes. Thus, oligo-FISH appears as a powerful tool not only for comparative karyotyping but also for the validation of genome assemblies.
DOI: 10.3389/fpls.2021.791303
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Alžběta Doležalová, Jaroslav Doležel, Kateřina Holušová, Eva Hřibová, Miroslava Karafiá..., Denisa Beránková
FRONTIERS IN PLANT SCIENCE 12: 791303, 2022
Klíčová slova: Cicer arietinum L., kabuli type, desi type, oligopainting FISH, chromosome identification, chromosome translocation
Abstrakt: Chickpea (Cicer arietinum L.) is one of the main sources of plant proteins in the Indian subcontinent and West Asia, where two different morphotypes, desi and kabuli, are grown. Despite the progress in genome mapping and sequencing, the knowledge of the chickpea genome at the chromosomal level, including the longrange molecular chromosome organization, is limited. Earlier cytogenetic studies in chickpea suffered from a limited number of cytogenetic landmarks and did not permit to identify individual chromosomes in the metaphase spreads or to anchor pseudomolecules to chromosomes in situ. In this study, we developed a system for fast molecular karyotyping for both morphotypes of cultivated chickpea. We demonstrate that even draft genome sequences are adequate to develop oligo-fluorescence in situ hybridization (FISH) barcodes for the identification of chromosomes and comparative analysis among closely related chickpea genotypes. Our results show the potential of oligo-FISH barcoding for the identification of structural changes in chromosomes, which accompanied genome diversification among chickpea cultivars. Moreover, oligo- FISH barcoding in chickpea pointed out some problematic, most probably wrongly assembled regions of the pseudomolecules of both kabuli and desi reference genomes. Thus, oligo-FISH appears as a powerful tool not only for comparative karyotyping but also for the validation of genome assemblies.
DOI: 10.3389/fpls.2021.791303
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Alžběta Doležalová, Jaroslav Doležel, Kateřina Holušová, Eva Hřibová, Miroslava Karafiá..., Denisa Beránková