Leptosphaeria maculans effector AvrLm4-7 affects SA- and ET-signalling and H2 O2 accumulation in Brassica napus

Nováková M., Šašek V., Trdá L., Krutinová H., Mongin T., Valentová O., Balesdent M.H., Rouxel T., Burketová L.
: , 2015

Klíčová slova: Leptosphaeria maculans, Brassica napus, AvrLm4-7
Abstrakt: To achieve host colonization, successful pathogens need to overcome plant basal defences. For this, (hemi)biotrophic pathogens secrete effectors that interfere with a range of physiological processes of the host plant. AvrLm4-7 is one of the cloned effectors from the hemibiotrophic fungus Leptosphaeria maculans 'brassicacae' infecting mainly oilseed rape (Brassica napus). While its mode of action is still unknown, AvrLm4-7 is strongly involved in L. maculans virulence. Here, we investigated the effect of AvrLm4-7 on plant defence responses in a susceptible cultivar of B. napus. Using two isogenic L. maculans isolates differing in the presence of a functional AvrLm4-7 allele (´a4a7´-absence and ´A4A7´-presence of the allele), the plant hormone concentrations, defence-related gene transcription and ROS accumulation were analysed in infected B. napus cotyledons. Various components of the plant immune system were affected. Infection with the ´A4A7´ isolate causes suppression of salicylic acid- and ethylene-dependent signalling, the pathways regulating an effective defence against L. maculans infection. Furthermore, ROS accumulation was decreased in cotyledons infected by the ´A4A7´ isolate. Treatment with an antioxidant agent, ascorbic acid, increased the aggressiveness of the ´a4a7´ L. maculans isolate but not that of the ´A4A7´ isolate. Together our results suggest that the increased aggressiveness of the ´A4A7´ L. maculans isolate could be caused by defects in ROS-dependent defence and/or linked to suppressed SA- and ET- signalling. This is the first study to bring insights into the manipulations of B. napus defence responses by an effector of L. maculans. This article is protected by copyright. All rights reserved.
Autoři z ÚEB: Lenka Burketová, Lucie Trdá