Platelet aggregation and anti-inflammatory effects of garden pea, desi chickpea and kabuli chickpea

Zia-Ul-Haq M., Khan M.A., Landa P., Kutil Z., Ahmed S., Qayum M., Ahmad S.

Klíčová slova: platelet aggregation, Garden pea, Desi chickpea, Kabuli chickpea, cardiovascular disease
Abstrakt: Inflammation is the natural body defense mechanism for the removal of injurious agents, necrosed cells and tissues from the body. This study was aimed to evaluate the anti-inflammatory and platelet aggregation effects of three medicinal plants of Pakistan. Methanolic extract of Garden pea inhibited arachidonic acid (AA)-induced platelet aggregation (IC50 = 35 μg/mL) and platelet activating factor (PAF)-induced platelet aggregation (IC50 = 38 μg/mL) in a dose dependent fashion. Methanolic extract of Desi chickpea inhibited arachidonic acid (AA) induced platelet aggregation (IC50 value = AA = 46 μg/mL) in dose dependent fashion while was found not active against PAF-induced platelet aggregation. Methanolic extract of Kabuli chickpea was found not active against both arachidonic acid (AA)-induced platelet aggregation and PAF-induced platelet aggregation. The best potential to inhibit in vitro COX-2 activity showed garden pea (Pisum sativum; the synthesis of PGE2 reduced by 92% in comparison with untreated control wells) followed by Desi chickpea (Cicer arietinum var; 87% inhibition) and Kabuli chickpea extracts (Cicer arietinum var; 65% inhibition). All extracts were tested at concentration 20 μg/mL. in COX-2 assay.The results indicate that if the same were happening in vivo, Garden pea, Desi chickpea and Kabuli chickpea could be useful as natural antithrombotic anti-inflammatory materials.
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