Comet assay in higher plants

Gichner, Tomáš
In Bioassays in Plant Cells for Improvement of Ecosystem and Human Health. Katowice : 123-130, 2003

Klíčová slova: DNA damage; genotoxicity
Abstrakt: Plants are exposed to various types of environmental xenobiotics, either deliberately as in the case of agricultural pesticides and plant growth regulators, or accidentally as compounds present in polluted air, soil or water. Assays to detect the genotoxicity of these xenobiotics are at present not available for most plant species. This limitation hampers or prevents the detection of the genotoxicity of environmental xenobiotics in plants growing, for example, on polluted soil. To overcome this limitation, a plant-based molecular assay - the COMET or Single Cell Gel Electrophoresis (SCGE) assay, can be applied to detect induced DNA damage (see reviews TICE et at, 2000; COLLINS, 2002). Although this technique has been primarily applied to animal cells, the incorporation of the Comet assay with plant tissues (KOPPEN, VERSCHAEVE, 1996; GICHNER, PLEWA, 1998) significantly extends the utility of plants in basic and applied studies in environmental mutagenesis. In theory, the Comet assay can be used to every plant species.
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