Effect of nano-hydroxyapatite and phosphate on thorium toxicity – Arabidopsis transcriptomic study

Landa P., Müller K., Přerostová S., Petrová Š., Moťková K., Vaněk T., Soudek P.

Klíčová slova: RNA-seq, stress response, deficiency, phytotoxicity, phosphorus
Abstrakt: Thorium (Th) is a ubiquitous element but elevated concentrations caused by anthropogenic or natural activities pose health and environmental risks. Nowadays, Th is obtained mainly as by-product during the mining and extraction of rare earth elements. However, due to its abundance in nature, which is higher than the abundance of uranium, Th is considered as a potential fuel for the reactors. Therefore, in this study, we analyzed the effect of thorium on Arabidopsis plants by RNA-seq profiling. Since Th and phosphorus form insoluble phosphates, we focused on the effect of Th in the presence/absence of phosphate in wild-type and mutants with altered phosphate homeostasis (management). Furthermore, we investigated the effect of hydroxyapatite nanoparticles, which are considered as material for in situ remediation, on Th toxicity. The highest number of DEGs was recorded in the combination of P absence and Th presence while the lowest in the presence of Th together with hydroxyapatite. Besides a high number of genes involved in stress response, Th also induced the transcription of genes involved in response to P starvation, indicating that Th decreased the availability of P. P deficiency caused by Th contributed to the phytotoxic effect of Th. Nano-hydroxyapatite significantly reduced Th toxicity. Nano-hydroxyapatite also served as a source of inorganic P for the plants, but caused deficiency of essential metals indicated by up-regulation of genes involved in their acquisition.
DOI: 10.1016/j.envexpbot.2023.105573
Autoři z ÚEB: Přemysl Landa, Kateřina Moťková, Karel Müller, Šárka Petrová, Sylva Přerostová, Petr Soudek, Tomáš Vaněk