Čeština
EnglishELECTROCHEMICAL REDUCTION OF 6-BENZYLAMINOPURINE AT MERCURY ELECTRODES AND ITS ANALYTICAL APPLICATION
Tarkowská, Danuše; Kotouček, M.; Doležal, Karel
COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS 68 [6]: 1076-1093, 2003
Klíčová slova: 6-Benzylaminopurine; Cytokinins; Electrochemistry
Abstrakt: The commercial exploitation of modern, in vitro plant micropropagation methods, featuring synthetic cytokinins as essential components of the cultivation media, is rapidly increasing. Thus, development of rapid, inexpensive and less labour-intensive methods for monitoring cytokinin levels could help to optimise media consumption and reduce costs. Therefore, we studied the electrochemical behaviour of the highly active and widely used cytokinin, 6-benzylaminopurine (BAP), in aqueous solutions by DC polarography, cyclic and differential pulse voltammetry and constant potential coulometry. The BAP molecule undergoes a six-electron irreversible reduction process that starts with four-electron reduction of the protonated pyrimidine skeleton. As a result of elimination of the amine from the side chain, the N1=C6 electrochemically active bond is re-established and the last two-electron step follows. The intermediates of constant potential electrolysis were identified using mass spectrometric analysis. The dissociation constant (pKa) of BAP was found, spectrophotometrically, to be 4.16. BAP concentrations were measured using two voltammetric techniques, fast-scan differential pulse (FSDPV) and adsorptive stripping voltammetry (AdSV). The relative standard deviations for these two methods were lower than 4.5% (c < 28.7 ng ml-1) and 1.2% (c < 20 ng ml-1), while the detection limits were 7.88 and 0.80 ng ml-1, respectively.
DOI:
Autoři z ÚEB: Danuše Tarkowská
COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS 68 [6]: 1076-1093, 2003
Klíčová slova: 6-Benzylaminopurine; Cytokinins; Electrochemistry
Abstrakt: The commercial exploitation of modern, in vitro plant micropropagation methods, featuring synthetic cytokinins as essential components of the cultivation media, is rapidly increasing. Thus, development of rapid, inexpensive and less labour-intensive methods for monitoring cytokinin levels could help to optimise media consumption and reduce costs. Therefore, we studied the electrochemical behaviour of the highly active and widely used cytokinin, 6-benzylaminopurine (BAP), in aqueous solutions by DC polarography, cyclic and differential pulse voltammetry and constant potential coulometry. The BAP molecule undergoes a six-electron irreversible reduction process that starts with four-electron reduction of the protonated pyrimidine skeleton. As a result of elimination of the amine from the side chain, the N1=C6 electrochemically active bond is re-established and the last two-electron step follows. The intermediates of constant potential electrolysis were identified using mass spectrometric analysis. The dissociation constant (pKa) of BAP was found, spectrophotometrically, to be 4.16. BAP concentrations were measured using two voltammetric techniques, fast-scan differential pulse (FSDPV) and adsorptive stripping voltammetry (AdSV). The relative standard deviations for these two methods were lower than 4.5% (c < 28.7 ng ml-1) and 1.2% (c < 20 ng ml-1), while the detection limits were 7.88 and 0.80 ng ml-1, respectively.
DOI: