Čeština
EnglishGamma-tubulins and Their Functions in Plant Cells
Binarová P., Cenklová V., Pochylová Ž., Dráberová E., Dráber P.
The Plant Cytoskeleton: a Key Tool for Agro-Biotechnology : 23-43, 2008
Klíčová slova: γ-Tubulin, microtubules, nucleation, plants, non-centrosomal
Abstrakt: Higher plants represent an important group of eukaryotes where discrete sites for microtubule nucleation are absent in all mitotic and meiotic cells, and the concept of dispersed microtubule-nucleation sites has been generally accepted. Moreover, even in cells containing centrosomes or spindle pole bodies, microtubules can nucleate non-centrosomally but molecular mechanisms behind the process are so far poorly characterized. γ- Tubulin is a ubiquitous phylogenetically conserved member of tubulin superfamily, present within the cells in various types of microtubuleorganizing centers and cytoplasmic protein complexes. Down regulation of γ-tubulin showed its essential role in non-centrosomal nucleation in plants. Plant γ-tubulin is localized with all microtubule arrays, and in nuclei. Protein complexes of γ-tubulin are present in soluble forms in cytoplasm and also with membranes. Association of γ-tubulin complexes with microtubules and dynamic membranes might ensure the flexibility of noncentrosomal microtubule nucleation. However the presence of various molecular forms of γ-tubulin suggests that, in addition to its nucleation role, additional roles for non- centrosomal γ-tubulin might exist.
DOI: https://doi.org/10.1007/978-1-4020-8843-8_2
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Žaneta Pochylová
The Plant Cytoskeleton: a Key Tool for Agro-Biotechnology : 23-43, 2008
Klíčová slova: γ-Tubulin, microtubules, nucleation, plants, non-centrosomal
Abstrakt: Higher plants represent an important group of eukaryotes where discrete sites for microtubule nucleation are absent in all mitotic and meiotic cells, and the concept of dispersed microtubule-nucleation sites has been generally accepted. Moreover, even in cells containing centrosomes or spindle pole bodies, microtubules can nucleate non-centrosomally but molecular mechanisms behind the process are so far poorly characterized. γ- Tubulin is a ubiquitous phylogenetically conserved member of tubulin superfamily, present within the cells in various types of microtubuleorganizing centers and cytoplasmic protein complexes. Down regulation of γ-tubulin showed its essential role in non-centrosomal nucleation in plants. Plant γ-tubulin is localized with all microtubule arrays, and in nuclei. Protein complexes of γ-tubulin are present in soluble forms in cytoplasm and also with membranes. Association of γ-tubulin complexes with microtubules and dynamic membranes might ensure the flexibility of noncentrosomal microtubule nucleation. However the presence of various molecular forms of γ-tubulin suggests that, in addition to its nucleation role, additional roles for non- centrosomal γ-tubulin might exist.
DOI: https://doi.org/10.1007/978-1-4020-8843-8_2
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Žaneta Pochylová