Hardening by abscisic acid of tobacco plantlets grown in vitro.

Pospíšilová J.
BIOLOGIA PLANTARUM 38: 605-609, 1996

Klíčová slova: ex vitro transfer, in vitro hardening, Nicotiana tabaccum, stomatal conductance, adaxial and abaxial epidermes
Abstrakt: Abstract Tobacco planflets were grown in vitro on Murashige and Skoog's medium with 2 % of saccharose. Addition of 0.01 mM abscisic acid (ABA) into the medium decreased stomatal conductance of the adaxial epidermis and especially the abaxial epidermis without negative effects on growth parameters. As a result the rate of water loss from ABA-treated plantlets taken out of cultivation vessels was slower than that of control plantlets. This could help their acclimation after transplantation to ex vitro conditions.
DOI: 10.1007/BF02890616
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