The use of mutagens to increase the efficiency of the androgenic progeny production in Solanum nigrum

Kopecký, David; Vagera, Jiří
BIOLOGIA PLANTARUM 49 [2]: 181-186, 2005

Klíčová slova: enhanced androgenesis; mutagenesis; induced haploidy
Abstrakt: The pollen embryogenesis in Solanum nigrum L. (2n=6x=72) was successfully induced in all experimental and treated variants. The significant stimulation of androgenesis (numbered according to frequency of androgenic responsive anthers) was observed after 10 mM and 20 mM ethylmethanesulfonate (EMS), 10 mM and 20 mM sodium azide (NaN3) and 0.2 mM N-nitroso-N-methylurea (MNU) treatment. The frequency of androgenesis on medium with sucrose was significantly higher than on medium with maltose. The androgenic regenerants were obtained from anthers collected from donor plants where survival of mutagenic treatment was less than 50 %. We obtained green haploid (3x), aneuploid (to 8x) and dihaploid (6x) plants. We explain the high frequency of aneuploids among androgenic plants by irregularities in cell divisions in microsporial calluses. The stimulation of androgenesis induced by mutagenic treatment can be explained by increasing of genetic heterogeneity (through site mutations) among cells inside anthers.
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